Protein post-translational modification positioning method
A post-translational modification and protein technology, applied in the field of proteomics and bioinformatics, can solve problems such as data confusion, protein variant accuracy to be discussed, missing information, etc., to achieve precise positioning improvement, efficient analysis and accurate structure identification Effect
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[0033] A method for protein post-translational modification localization, comprising the following steps:
[0034] (1) According to the sequence and modification information in the standard protein sequence database, generate the theoretical primary mass spectrometry database and secondary mass spectrometry database of all corresponding protein variants;
[0035] (2) compare the experimental primary mass spectrum with the theoretical primary mass spectrum, and find all candidate protein variants that match the primary mass spectrum;
[0036](3) Matching the experimental secondary mass spectrometry data with the theoretical secondary mass spectrometry data of all candidate protein variants to obtain the matching fragment ion information of each candidate protein variant;
[0037] (4) Select a candidate protein variant, if it does not contain post-translational modification, then no post-translational modification localization scoring; if it contains post-translational modificat...
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