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A micro-ecological preparation for improving milk yields of dairy cows

A microecological preparation and milk production technology, which is applied in the direction of medical preparations containing active ingredients, animal feed, diseases, etc., can solve the problems of microecological preparations, such as pertinence and low specificity, and achieve the maintenance of intestinal tract Micro-ecological balance, strong specificity, and the effect of improving nutrient utilization

Inactive Publication Date: 2015-08-05
XUZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of probiotics that can improve the milk production of dairy cows, and solve the problem that the existing probiotics are not targeted and specific

Method used

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  • A micro-ecological preparation for improving milk yields of dairy cows
  • A micro-ecological preparation for improving milk yields of dairy cows

Examples

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Embodiment 1

[0019] Example 1: Use culture-free technology combined with statistical methods to screen the dominant bacteria in the rumen of high-yielding dairy cows, use pure culture technology to obtain pure strains of dominant bacteria, and combine them into microecological preparations through fermentation and culture; prepare according to the following steps :

[0020] (1) Sequencing: extract DNA from rumen liquid of Holstein dairy cows for PCR amplification, and obtain rumen microbial community components of dairy cows through high-throughput sequencing and taxonomic analysis of a genome sequencer; the genome sequencer expresses Miseq in English;

[0021] (2) Screening: The rumen-dominant bacteria of high-yield dairy cows, namely Bacteroides and Ruminococcus, were screened out through statistical analysis;

[0022] (3) Separation and purification of bacterial strains: extract cow rumen fluid, use the Henggate rolling tube method to cultivate rumen microorganisms, select Bacteroides a...

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Abstract

The present invention discloses a micro-ecological preparation for improving milk yields of dairy cows, and belongs to the preparation for improving the milk yields of the dairy cows. The micro-ecological preparation uses a method combining statistical methods with culture-independent technology to screen dominant bacteria in rumens of high-yielding dairy cows; pure strains are obtained from the dominant bacteria by using pure culture technology; and the micro-ecological preparation is prepared through fermenting, culturing, combining and mixing the pure strains. The micro-ecological preparation is prepared by following specific steps: (1) sequencing, (2) screening, (3) separating and purifying strains, (4) culturing, (5) fermenting (6) mixing and (7) packaging. The micro-ecological preparation contains Bacteroidaceae and Ruminococcus helpful for decomposing polysaccharide and cellulose, can improve Bacteroidaceae and Ruminococcus contents in rumen bacteria of the high-yielding dairy cows in order to ensure the advantage position and improve the utilization of the cellulose and the polysaccharide, thus improving absorption and utilization of feed nutrient components for the dairy cows, increase the milk yields and bring economic benefits. The Bacteroidaceae can help the host absorb the polysaccharide, improve nutrition utilization rates and host's immunity, and maintain gastrointestinal micro-ecological balance. The micro-ecological preparation has a strong pertinence and specificity.

Description

technical field [0001] The invention relates to a preparation for increasing milk production of dairy cows, in particular to a microecological preparation for increasing milk production of dairy cows. Background technique [0002] Microecological preparation is a green and safe feed additive with no toxic side effects and no drug resistance. It can adjust or maintain the microecological balance in the gastrointestinal tract of animals, enhance the body's immunity, and promote the digestion and absorption of nutrients. [0003] At present, the feed microecological preparations commonly used by ruminants at home and abroad include bacteria such as lactic acid bacteria (Lactobacillus, Bifidobacterium, etc.), Bacillus (Bacillus subtilis, Bacillus cereus, etc.), Aspergillus (Aspergillus niger, etc.) , Aspergillus oryzae) and yeasts (beer yeast, and petroleum yeast and their cultures) and other single and complex bacterial preparations. These probiotics sold in the market are not...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A61K35/741A61P15/14
Inventor 季烽王秀颖温洪宇
Owner XUZHOU NORMAL UNIVERSITY
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