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Preparation method of AIDS (acquired immune deficiency syndrome) virus affinity adsorbent

A technology for HIV and virus adsorption, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as ligands are easy to fall off, and achieve improved safety, strong ligand binding, The effect of reducing the chance and rate of shedding

Inactive Publication Date: 2015-07-29
WUHAN RUIFA MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defect that the ligands on the HIV-affinity adsorbent prepared in the prior art are easy to fall off, and provide a preparation of an HIV-affinity adsorbent that is easy to operate and can greatly improve the binding firmness of the ligands. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of HIV affinity adsorbent comprises the following steps:

[0020] 1) Prepare HIV affinity ligand CD4 molecules and glass microspheres as carriers;

[0021] How to prepare the above-mentioned CD4 molecule is a published prior art, and will not be described in detail in this application.

[0022] Surface pretreatment of glass microspheres: wash glass microspheres with an average diameter of 85 μm with 65% concentrated nitric acid for 10 minutes, then wash with distilled water (3 times with deionized water, 2 times with distilled water, 2 minutes each time), put them in a 37°C oven drying.

[0023] 2) Activate the surface groups of glass microspheres: Take double-distilled water, acetone (analytical grade), silanized coupling agent (γ-aminopropyltriethoxysilane (APTES) in a volume ratio of 15:280:6 Prepare an APTES activation solution, and then immerse the glass microspheres in it for 2 hours; wash the glass slide with acetone 5 times (5min / time), ...

Embodiment 2

[0029] The preparation method of HIV affinity adsorbent comprises the following steps:

[0030] 1) Prepare HIV affinity ligand CXCR-4 molecules and glass microspheres as carriers;

[0031] How to prepare the above-mentioned CXCR-4 is a published prior art, and will not be described in detail in this application.

[0032] Surface pretreatment of glass microspheres: wash glass microspheres with an average diameter of 85 μm with 65% concentrated nitric acid for 10 minutes, then wash with distilled water (3 times with deionized water, 2 times with distilled water, 2 minutes each time), put them in a 37°C oven drying.

[0033] 2) Activate the surface groups of glass microspheres: Take double-distilled water, acetone (analytical grade), silanized coupling agent (γ-aminopropyltriethoxysilane (APTES) in a volume ratio of 15:280:6 Prepare an APTES activation solution, and then immerse the glass microspheres in it for 2 hours; wash the glass slide with acetone 5 times (5min / time), and...

Embodiment 3

[0039] The preparation method of HIV affinity adsorbent comprises the following steps:

[0040] 1) Preparation of HIV affinity ligand and glass microspheres as carrier;

[0041] ①The ligand is: a 1:1:1:1 mixture of CD4, CXCR-4, CCR-5 and gp120 antibodies;

[0042] How to prepare the above-mentioned ligands is a published prior art, and will not be described in detail in this application.

[0043] ② Surface pretreatment of glass microspheres: wash glass microspheres with an average diameter of 85 μm with 65% concentrated nitric acid for 10 minutes, then wash with distilled water (3 times with deionized water, 2 times with distilled water, each time for 2 minutes), and put them in 37°C Oven dry.

[0044] 2) Activate the surface groups of glass microspheres: Take double-distilled water, acetone (analytical grade), silanized coupling agent (γ-aminopropyltriethoxysilane (APTES) in a volume ratio of 15:280:6 Prepare an APTES activation solution, and then immerse the glass microsp...

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PUM

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Abstract

The invention discloses a preparation method of an AIDS (acquired immune deficiency syndrome) virus affinity adsorbent. According to the method, amino acid is utilized to process virus adsorption microspheres connected with ligands, the ligands on the virus adsorption microspheres are combined more firmly, so that the probability and the speed of falling of the AIDS virus affinity adsorbent are reduced, and the safety of the AIDS virus affinity adsorbent during applying is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of an HIV-affinity adsorbent. Background technique [0002] HIV (AIDS virus) is officially named Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV) and belongs to the Lentiviridae subfamily of Retroviridae. [0003] The prior art discloses an HIV affinity adsorption column and its preparation method and application. In this method, the HIV affinity protein is connected to microspheres, and the affinity protein is specifically bound to HIV, so as to remove the virus in the blood. HIV purpose. [0004] At present, the commonly used microspheres for preparing this type of adsorbent are agarose microspheres, polyvinyl alcohol microspheres, chitosan microspheres, etc. The ligands connected to the microspheres include receptor CD4 molecule (CD4), gp120 antibody, auxiliary Receptor CXC chemokine receptor 4 (CXCR-4) or CC chemokine receptor 5 (CCR-5), etc...

Claims

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Application Information

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IPC IPC(8): B01J20/22B01J20/28B01J20/30
Inventor 王业富韩振伟李卫刘婧
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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