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Stress-induced bowel disorder-relieving agent comprising specific lactobacillus gasseri strain or treatment product thereof

A technology of Lactobacillus gasseri, stress, used in the field of mitigants

Inactive Publication Date: 2015-07-22
ASAHI GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Daisuke Sawada et al., The 10th Symposium on Lactic Acid Bacteria (2011), the Netherlands, Federation of European Microbiological Societies (FEMS) and Netherlands Society for Microbiology (NVvM) (Non-Patent Document 2), describe the effect on irritable bowel Syndrome (IBS) patients were given CP2305 live bacteria, but there is no record for the relief of diarrhea symptoms

Method used

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  • Stress-induced bowel disorder-relieving agent comprising specific lactobacillus gasseri strain or treatment product thereof
  • Stress-induced bowel disorder-relieving agent comprising specific lactobacillus gasseri strain or treatment product thereof
  • Stress-induced bowel disorder-relieving agent comprising specific lactobacillus gasseri strain or treatment product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142]

[0143] Inoculate Lactobacillus gasseri CP2305 strain (number of bacteria 10 3 a ~ 10 4 ), cultured at 37°C for 19 hours.

[0144] After culturing for 19 hours, this 15-ml polypropylene test tube with an inner diameter of 15 mm was inserted into a sufficient amount of ice above the liquid level in the test tube, rapidly cooled (on ice), and kept for 5 hours. This cultivation and cooling were repeated 80 times, and a strain with high low temperature tolerance was bred.

[0145] In this specification, "on ice" refers to a state in which 10 ml of culture solution is placed in a 15 ml test tube made of polypropylene with an inner diameter of 15 mm, and the test tube is cooled by inserting a sufficient amount of ice above the liquid level in the test tube. .

[0146] In addition, the composition of the "food additive medium" used in this specification is as follows (Table 1).

[0147] [Table 1]

[0148] Composition of food additive medium

[0149] compositi...

Embodiment 2

[0157]

[0158] It should be noted that, as a method for producing a low-temperature tolerant strain, for example, the CP2305 strain tends to have a reduced survival rate at temperatures below 10°C. Therefore, as a method for producing a low-temperature tolerant strain, the target strain is cultured in a culture solution (for example: a food additive medium) at a moderate culture temperature (for example: about 37°C) and for a moderate culture time (for example: about 19 hours). After culturing, the culture solution is exposed for a moderate time at a temperature below 10°C, preferably below 7°C, more preferably below 5°C, more preferably below 3°C, below 2°C, or below 1°C (for example: 5 hours), and, if necessary, by repeating their cultivation and exposure to low temperature, strains resistant to low temperatures at the target temperature were produced.

Embodiment 3

[0160] SD male rats (4-week-old, manufactured by Charles River Laboratories) were bred under a 12-hour light-dark cycle (lighting on for 12 hours from 8 o'clock) for 1 week, and then allowed to freely ingest the test bacterial cell mixture bait for 3 weeks as follows.

[0161] a) CRF-1 powder bait from Oriental Yeast Co., Ltd. was used as bait.

[0162] b) The number of bacteria contained in 25 g of the above-mentioned bait (CRF-1) is 2.0×10 10 The freeze-dried powder (0.25 g of the freeze-dried powder was compounded with respect to the bait CRF-1: 25 g) of each test cell was used as the test cell compounded bait.

[0163] c) Allow them to freely ingest the mixed bait of the test bacteria for 3 weeks.

[0164] d) The food intake of the bacteria in this test is estimated to be about 2.0×10 10 pcs / day.

[0165] e) Let 19 animals in each group ingest.

[0166] After 3 weeks, a CRF (Corticotropin Releasing Factor, manufactured by Anaspec, USA) solution was intraperitoneally ad...

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Abstract

The present application provides: Lactobacillus gasseri strain CP2305 (FERM BP-11331), Lactobacillus gasseri strain CP2305s (NITE BP-1405), or a variant strain (e.g., a psychrotolerant variant strain) of Lactobacillus gasseri strain CP2305 (FERM BP-11331) or strain CP2305s; a method for producing the variant strain; a stress-induced bowel disorder-relieving agent which is characterized by comprising, as an active ingredient, the variant strain, a treatment product of cells of the variant strain or a mixture thereof; and a food additive, an animal additive, an animal feed and a pharmaceutical composition, each of which contains the relieving agent.

Description

technical field [0001] The present invention relates to a reducer (or inhibitor) of excessive water secretion in the intestinal tract of a subject, or a pressure-sensitive Relief agents (or inhibitors) of symptoms of bowel disorders, especially stress diarrhoea. [0002] The present invention also relates to a novel cold-tolerant Lactobacillus gasseri strain and a production method thereof. [0003] The present invention further relates to a reliever (or inhibitor) of stress bowel disorder comprising the above-mentioned cold-tolerant Lactobacillus gasseri strain or a processed product thereof as an active ingredient. [0004] The present invention also relates to food additives or animal feed additives, or food or pharmaceutical compositions containing the above-mentioned alleviating agent (or inhibitor). Background technique [0005] As for prior art documents showing the possibility that lactic acid bacteria of the genus Lactobacillus (Lactobacillus) are effective for st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A23K1/16A23L1/30A61P1/04A61P1/12A61P25/02C12N1/20C12R1/245A23K20/00A23L2/52A23L33/135
CPCC12N1/20A23K10/16A23K10/18A23L33/135A61K35/747A23V2002/00A61K2035/115A61P1/12C12R2001/225C12N1/205A23V2200/32A23V2400/145A23L2/52A61K9/0056
Inventor 泽田大辅藤原茂
Owner ASAHI GRP HLDG LTD
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