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Kit applied to embryonic development quality evaluation and use method

A technology for embryo development and quality assessment, applied in the field of medical testing, can solve the problem of lack of objective evaluation indicators for embryo development, and achieve the effects of standardized testing methods, high specificity, and small sample size.

Active Publication Date: 2015-07-22
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that the existing assisted reproductive technology lacks objective evaluation indicators for embryo development, the present invention provides a kit and application method for evaluating the quality of embryo development

Method used

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  • Kit applied to embryonic development quality evaluation and use method
  • Kit applied to embryonic development quality evaluation and use method
  • Kit applied to embryonic development quality evaluation and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kit for evaluating the quality of embryonic development, a biomarker detection reagent for determining the different expression levels of sperm long non-coding RNA molecule LA16c390E6.5, and evaluating the quality of embryonic development through the expression level of LA16c390E6.5; said LA16c390E6 The gene sequence of .5 is: SEQID NO:1.

Embodiment 2

[0030] A kit for evaluating the quality of embryonic development, a biomarker detection reagent for determining the different expression levels of sperm long non-coding RNA molecule LA16c390E6.5, and evaluating the quality of embryonic development through the expression level of LA16c390E6.5; said LA16c390E6 The gene sequence of .5 is: SEQID NO:1.

[0031] The kit includes positive control substance, reverse transcription system, fluorescent quantitative PCR reaction system, SYBR Green reagent and LA16c390E6.5 specific primer probe.

Embodiment 3

[0033] A kit for evaluating the quality of embryonic development, a biomarker detection reagent for determining the different expression levels of sperm long non-coding RNA molecule LA16c390E6.5, and evaluating the quality of embryonic development through the expression level of LA16c390E6.5; said LA16c390E6 The gene sequence of .5 is: SEQID NO:1.

[0034] The kit includes positive control substance, reverse transcription system, fluorescent quantitative PCR reaction system, SYBR Green reagent and LA16c390E6.5 specific primer probe.

[0035] The positive control substance is the cDNA extracted from the RNA of the normal sperm sample.

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Abstract

The invention provides a kit applied to embryonic development quality evaluation and a use method. The kit is a biomarker detection reagent used for determining different expression levels of a sperm long non-coding RNA molecule LA16c390E6.5, and the embryonic development quality is evaluated by the expression levels of the LA16c390E6.5; and a gene sequence of the LA16c390E6.5 is shown as SEQ ID No. 1. By clinically utilizing sperm molecular marker long non-coding RNA molecule LA16c390E6.5, the embryonic development potency can be accurately predicted, and guiding significance is provided for curative effect detection and assisted reproduction manner selection.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a kit for evaluating the quality of embryonic development and a method for using it. Background technique [0002] The pros and cons of embryo quality assessment methods are directly related to the clinical pregnancy outcome of assisted reproduction. At present, in the evaluation methods of embryo quality, the most widely used method is the morphological evaluation method of scoring the morphological characteristics of gamete embryos at each stage. It is mainly evaluated by observing indicators such as embryo development and fragmentation, and lacks objective evaluation indicators. [0003] The document "Research Progress on Sperm RNA" ("Carcinoma, Aberration, Mutation", 2011, Volume 23, Issue 5) discloses that sperm RNA plays a very important role in the process of sperm motility, capacitation, fertilization and embryonic development. effect. Sperm RNA c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 许文明王可杨明陈艳廖惠娟孙华钦
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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