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A strain of Lactobacillus plantarum ab-2 with broad-spectrum antibacterial properties and its application

A technology of Lactobacillus plantarum and broad-spectrum antibacterial, applied in the direction of medical preparations containing active ingredients, applications, bacteria, etc., can solve the problems of drug residue treatment effect, pathogenic bacteria drug resistance, bacterial pollution, etc., and improve intestinal The effect of the internal microbial environment

Active Publication Date: 2016-02-03
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Food microbial contamination is mainly bacterial contamination, especially the common pathogenic single-cell Listeria, Staphylococcus aureus, Escherichia coli and Salmonella typhi can cause foodborne diseases, and now this problem has caused Public attention, common chemical drug treatment methods are prone to defects such as drug resistance of pathogenic bacteria, drug residues and poor treatment effect

Method used

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  • A strain of Lactobacillus plantarum ab-2 with broad-spectrum antibacterial properties and its application
  • A strain of Lactobacillus plantarum ab-2 with broad-spectrum antibacterial properties and its application
  • A strain of Lactobacillus plantarum ab-2 with broad-spectrum antibacterial properties and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1: Determination of L.plantarum AB-2 antibacterial activity

[0081] The preparation method of the fermentation supernatant of Lactobacillus plantarum: Inoculate the preservation solution of Lactobacillus plantarum AB-2 and other Lactobacillus plantarum preserved in the cryopreservation tube into 5mL of MRS liquid medium, shake and mix well, 37 Cultivate at constant temperature for 24 hours at ℃, continuously activate three generations with 2% inoculation amount, take the fermentation liquid of the third generation Lactobacillus plantarum and centrifuge at 4000×g for 10 minutes, take the supernatant, filter the supernatant through a 0.22 μm micro-membrane, and store in a -20°C refrigerator Save for later.

[0082] Indicator bacteria: Escherichia coli O157:H7 (EscherchiacoliO157:H7), Salmonella typhimurium ATCC13311, Shigella flexneri CMCC51592, Staphylococcus aureus ATCC12600, Listeria monocytogenes ATCC15313, etc. Five strains of intestinal pathogenic bacte...

Embodiment 2

[0092] Example 2: Gastrointestinal fluid tolerance of L. plantarum AB-2

[0093] After sterilizing the PBS, adjust the pH value to 2.5 with 1mol / L HCL, add 3.0mg / ml pepsin (Sigma, USA), filter and sterilize with a 0.22μm microporous membrane to make a simulated artificial gastric juice; sterilize the PBS Finally, adjust the pH value to 8.0 with 0.1mol / L NaOH, add 0.1wt% trypsin (Sigma, the United States) and 1.8wt% ox bile salt (DIFCO, Canada), filter and sterilize with a 0.22 μm microporous membrane, and prepare Artificially simulated pancreatic juice.

[0094] The re-screened bacterial strains in Example 1 (31 strains of Lactobacillus plantarum involved in Table 3) were activated and cultivated for two generations, washed twice by centrifugation, collected thalline, and got 0.5ml re-screened bacterial liquid Add it into 4.5mL of simulated artificial gastric juice (pH2.5), digest at 37°C for 3h, and count and determine the number of viable bacteria by MRS agar medium pouring...

Embodiment 3

[0103] Example 3: L. plantarum AB-2 bile salt tolerance

[0104] Insert the fermented liquid of activated L.plantarum AB-2 (preparation method as in Implementation 1) by 1wt% inoculum size into MRS liquid medium containing bovine bile salt (0.3wt% Oxgall+0.2wt% sodium thioglycolate) , with MRS without ox bile salt (DIFCO, Canada) as the control. The strain was cultured in a water bath at 37°C, and the OD value was measured at 620nm by sampling every hour until the OD value increased by 0.3 units. The ability of the test strains to tolerate bile salts was evaluated by the length of the lag time, and the difference in the time required for the OD value of the strains in the test group and the blank group to increase by 0.3 units was the lag time (LT, lagtime) (Table 5).

[0105] Delay time (h) of table 5L.plantarumAB-2 in bile salt solution

[0106]

[0107] It can be seen from Table 5 that the delay time of L.plantarum AB-2 is 0.23h. According to the judgment method of Gil...

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Abstract

The invention discloses a plant lactobacillus with broad-spectrum antibacterial properties, and the bacterium is named Lactobacillus? plantarum? AB-2, has been preserved in the General Microorganism Culture Collection Center of the China Microbiology Culture Collection Management Committee on September 15, 2014, and the preservation number is CGMCC? No. 9654. Plantarum lactobacillus of the present invention shows stronger broad-spectrum antibacterial characteristic under the condition of MRS liquid culture medium culture, and this bacterial strain has good probiotic characteristic, its MRS fermentation supernatant is passed through different pH, catalase, Pepsin and trypsin can still show good broad-spectrum antibacterial properties after treatment, which finally proves that it has good broad-spectrum antibacterial properties and the ability to survive in the human intestinal tract.

Description

technical field [0001] The invention relates to a plant Lactobacillus strain (Lactobacillus plantarum AB-2) isolated from Sichuan pickles and the application of the strain in fermented milk products and other health care foods using the strain as a starter or additive. technical background [0002] With the improvement of public health and safety awareness, more and more people pay attention to food safety issues, because food safety problems may cause major economic losses and seriously endanger human health and social security. Among them, the problem of food microbial contamination is one of the main problems of food safety. Food microbial contamination is mainly bacterial contamination, especially the common pathogenic single-cell Listeria, Staphylococcus aureus, Escherichia coli and Salmonella typhi can cause foodborne diseases, and now this problem has caused The attention of the public is that common chemical drug treatment methods are prone to defects such as drug r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P31/10A23L3/3571A23L33/135A23K20/00C12R1/25
CPCA23L3/3571A61K35/747C12N1/205C12R2001/25A61K2300/00Y02A50/30
Inventor 陈永福张和平李常坤
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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