Culture method and culture system of haematococcus pluvialis species

Abstract

The invention discloses a culture method of haematococcus pluvialis species. The culture method comprises the following steps: 1) heating haematococcus pluvialis species culture water to be 60 DEG C or above in a solar heating mode; 2) rinsing a haematococcus pluvialis species culture device by using the hot water obtained in the step 1); 3) discharging out the rinsing water, further filling the hot water obtained in the step 1) into the haematococcus pluvialis species culture device, when the water is naturally cooled to be less than 28 DEG C, preparing a culture medium in the haematococcus pluvialis species culture device, and inoculating haematococcus pluvialis species to start the species culture process. The applicant of the invention finds that when the haematococcus pluvialis species culture system is sterilized by using the hot water generated by using the solar energy, and meanwhile the cooled solar hot water is used as the haematococcus pluvialis species culture water, not only is the sterilization requirement on the haematococcus pluvialis species culture system met, but also the adverse influence to the haematococcus pluvialis species caused by biological contamination can be effectively controlled.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for cultivating Haematococcus, in particular to a method for cultivating Haematococcus pluvialis and a corresponding culture system. Background technique [0002] Haematococcus pluvialis (H. pluvialis) belongs to Chlorophata (Chiorophata), Chlorophyceae (Chiorophyceae), Volvocales (Volvocales), Haematococcaceae (Haematococcaceae), Haematococcus (Ag). In a specific ecological environment, it can form chlamydospores and accumulate a large amount of astaxanthin. It is the organism most capable of synthesizing and accumulating astaxanthin in a large amount in nature, and it is also the organism with the highest natural astaxanthin content in nature. Astaxanthin is a biological antioxidant, which has a strong ability to remove free radicals, protect cells and tissues from lipid oxidation and peroxidation damage, and has broad applications in human functional food, medici...

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Problems solved by technology

[0004] During the cultivation of Haematococcus pluvialis species, the effective measure for the prevention and control of biological pollution is to disinfect the algae culture system and the culture water before inoculation. The usual disinfection method is chemical disinfection, but chemical disinfection methods usually have disinfection problems. Thoroughly and additionally bring in chemical ion components that have nothing to do with the cultivation of algae, cause pollutants to be resistant to disinfectants after long-term use, and affect the normal growth of Haematococcus pluvialis, etc.

At present, the ideal disinfection method is thermal disinfection, that is, heating the algae cultivation system and water for cultivation; the way to obtain heat resources is generally electric heating, coal-fired boilers, etc., which usually have high energy consumption, high cost, and environmental impact. There are disadvantages such as pollution effects

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