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Method for applying dual high-resolution melting curve technology to detect Bartonella

A high-resolution melting and Bartonella technology, applied in the field of applying dual high-resolution melting curve technology to detect Bartonella, to achieve the effects of high sensitivity, improved sensitivity and good stability

Inactive Publication Date: 2015-07-15
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the simultaneous detection of Bh and Bq pathogens using dual HRM technology at home and abroad.

Method used

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  • Method for applying dual high-resolution melting curve technology to detect Bartonella
  • Method for applying dual high-resolution melting curve technology to detect Bartonella
  • Method for applying dual high-resolution melting curve technology to detect Bartonella

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Embodiment 1

[0029] Embodiment 1 Application of double high-resolution melting curve technology to detect the method of Bartonella

[0030] 1 Materials and methods

[0031] 1.1 Strain and biological sample DNA

[0032]Bartonella genomic DNA includes B.henselae (Bh) ATCC49882, B.quintana (Bq) ATCC VR-358, B.vinsonii subsp. .berkhoffii) ATCC 51672, Bacilliformis (Bb), Bartonella (B.clarridgeiae, Bc) ATCC 51734, Bartonella (B.koehlerae, Bk) ATCC 700693, ATCC35685, Vinson Bartonella subsp.vinsonii (B.vinsonii subsp.vinsonii, Bvv) ATCC VR-152, Elizabeth Bartonella (B.elizabethae, Be) ATCC 49927, Graham Bartonella (B.grahamii, Bg) ATCC 700132 and Daozhi Bartonella (B.doshiae, Bd) ATCC 700133; human, cat, rabbit, dog, sheep and tick genome DNA were all provided by the Vector Biological Control Office of the Institute of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention.

[0033] Other bacterial genomic DNAs include Streptococcus pneumoniae, Haemophilu...

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Abstract

The invention provides a method for applying a dual high-resolution melting curve technology to detect Bartonella. By the adoption of the method, Bartonella henselae and Bartonella Quintana can be simultaneously and quickly detected, and effective measures are provided for early-stage rapid diagnosis, monitoring, epidemiological investigation and the like of a series of diseases including endocarditis caused by the Bartonella henselae and Bartonella Quintana.

Description

technical field [0001] The invention relates to the field of molecular biology detection of Bartonella, in particular to a method for detecting Bartonella by applying double high-resolution melting curve technology. Background technique [0002] Infective endocarditis (IE) is a serious life-threatening disease with complex clinical manifestations and difficult diagnosis. In recent years, although the level of prevention and treatment has been greatly improved, the morbidity and mortality are still relatively high. At present, studies have found that there are more and more types of Bartonella spp. that infect human endocarditis. Eight species of Bartonella have been reported, among which B. henselae (Bh) and Bartonella quintana (B.quintana, Bq) mainly, these two pathogens are Gram-negative, oxidase-negative, demanding nutritional conditions, facultative intracellular parasitic aerobic bacteria, mainly parasitic in humans, cats, dogs and rodents In animals and other vascula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689C12Q1/686
Inventor 栗冬梅刘起勇陈忠科刘云彦王震杜鹏程宋秀平
Owner ICDC CHINA CDC
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