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Method for detecting sulfonamide antibiotic residues in milk

A detection method, the technology of sulfonamides, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost and cumbersome steps, and achieve the effect of high accuracy, less dosage and simple operation

Active Publication Date: 2015-07-08
YUNNAN FANGYUAN TECH
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Problems solved by technology

[0006] In order to overcome the shortcomings and deficiencies of the above-mentioned prior art sulfonamide antibiotic detection method with high cost, cumbersome steps and staying in the field of animal food detection, the object of the present invention is to provide a method for the detection of sulfa antibiotic in milk

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  • Method for detecting sulfonamide antibiotic residues in milk

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Embodiment

[0017] A method for detecting residues of sulfonamide antibiotics in milk, the steps comprising:

[0018] (1) Antibiotic-free milk is used as a blank sample and a sample to be tested, and the centrifugal reagent acidified acetonitrile is added to mix at a volume ratio of 1:2, and then centrifuged; after centrifugation, the centrifugal supernatant is adjusted with 1-10% dilute sulfuric acid to make Its pH value is 3-5, add a metal salt solution with a concentration of 0.5-1.5g / L and centrifuge supernatant, mix evenly and let it stand for 10 minutes;

[0019] (2) The instrument used is Shimadzu LC-20AT high performance liquid chromatograph, the chromatographic column specification is Shim-pack VP-ODS (150 × 4.6mm, 5 μ m), SPD-20A ultraviolet detector, detection wavelength 270nm (adopted The maximum absorption wavelength of sulfa drugs is determined to improve sensitivity), column temperature is 40°C, mobile phase: 0.5v / v% acetic acid solution: acetonitrile:methanol=75:20:5 (V:V)...

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Abstract

The invention relates to the technical field of food safety detection, in particular to a method for detecting sulfonamide antibiotic residues in milk. The method includes the steps that antibiotic-free milk serving as a blank sample and a sample to be detected are added in and mixed with centrifugal reagent acidized acetonitrile in the volume ratio of 1:2, and separation centrifuging is conducted; after centrifuging, 1-10% dilute sulphuric acid is used for adjusting the pH value of supernate to be 3-5, and a metal salt solution with the concentration being 0.5-1.5g / L is added to be evenly mixed with the supernate and stands still for 10 min; chromatographic determination is carried out, peak area corresponding to separated residual sulfonamide antibiotic is calculated, quantitative computation is carried out according to a standard curve, and the content of the residues is obtained. The use quantity of required samples and various reagents is small, environment harm is little, the speed of the process is detected, accuracy is high, operation is easy, and consumed time is short.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for detecting residues of sulfonamide antibiotics in milk. Background technique [0002] Milk is rich in minerals, calcium, phosphorus, iron, zinc, copper, manganese, molybdenum. The most rare thing is that milk is the best source of calcium for the human body, and the ratio of calcium to phosphorus is very appropriate, which is conducive to the absorption of calcium. There are at least 100 kinds of complex types, and the main components are water, fat, phospholipids, protein, lactose, inorganic salts, etc. [0003] Milk is a nutritious product suitable for all ages. If milk contains antibiotics, for long-term drinkers, it is undoubtedly equivalent to taking small doses of antibiotics for a long time. People who are allergic to antibiotics will have allergic reactions after taking milk with residual antibiotics. Even for normal drinkers, some conditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孙毅孙靖函蒋文清
Owner YUNNAN FANGYUAN TECH
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