Chloral hydrate permeabilization flaking method in microscopic identification of TCM

A microscopic identification, chloral hydrate technology, applied in the preparation of test samples, etc., can solve the problems of teachers and students, easy to blacken slides, low efficiency, etc., and achieve strong practical value, economic and social benefits Huge, wide-ranging effects

Inactive Publication Date: 2015-07-08
HENAN UNIV OF CHINESE MEDICINE
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the long-term practice, the shortcomings of this traditional permeable method have gradually emerged.
The use of alcohol lamps in the experiment has the danger of open flames, the temperature is difficult to control during film production, uneven heating is likely to occur, the powder is scorched, easy to generate air bubbles, and the flame of the alcohol lamp is easy to blacken the glass slides, etc., which greatly affects the microscope. clear observation of form
Chloral hydrate emits toxic gas when heated, is poisonous and irritating
Especially in the experimental teaching of Chinese medicine colleges and universities, if many people use it frequently in a poorly ventilated classroom for a long time, it will cause harm to teachers and students, and it is not easy to control, low in efficiency, and poor in safety.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chloral hydrate permeabilization flaking method in microscopic identification of TCM
  • Chloral hydrate permeabilization flaking method in microscopic identification of TCM

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the specific implementation of the present invention, at first heating equipment is installed, and then the chloral hydrate permeation film production in the traditional Chinese medicine microscopic identification is completed according to the following steps:

[0019] (1) Prepare chloral hydrate solution: dissolve chloral hydrate with water to form a chloral hydrate solution with a mass concentration of 30%;

[0020] (2) Prepare the mixture of Paeoniae Alba powder and chloral hydrate solution: add 0.1g of Paeoniae Alba powder into 2ml of chloral hydrate solution with a mass concentration of 30% to make a mixture;

[0021] (3) Heat and permeabilize the mixture: put the mixture in a container, put it in the heating tank of the closed heating zone of the heating equipment or in the open heating zone, start the heating equipment, preheat to 100°C first, and then raise the temperature to 125°C ℃, heating for 6 minutes to realize the permeabilization of the mixture;

[0...

Embodiment 2

[0024] In the specific implementation of the present invention, first install the heating equipment, and then perform the permeabilization film production according to the following steps:

[0025] (1) Prepare chloral hydrate solution: dissolve chloral hydrate with water to form a chloral hydrate solution with a mass concentration of 35%;

[0026] (2) Prepare a mixture of rehmannia leaf powder and chloral hydrate solution: add 0.2g rehmannia leaf powder to 2ml of chloral hydrate solution with a mass concentration of 35% to make a mixture;

[0027] (3) Heat and permeabilize the mixture: put the mixture in a container, put it in the heating tank of the closed heating zone of the heating equipment or in the open heating zone, start the heating equipment, preheat to 105°C first, and then raise the temperature to 130°C ℃, heating for 7 minutes to realize the permeabilization of the mixture;

[0028] (4) Preparation after permeabilization: drop 0.07ml of the permeabilized mixture i...

Embodiment 3

[0030] In the implementation of the present invention, heating equipment is firstly installed, and then permeabilization is performed according to the following steps:

[0031] (1) Prepare chloral hydrate solution: dissolve chloral hydrate with water to form a chloral hydrate solution with a mass concentration of 40%;

[0032] (2) Prepare a mixture of yam powder and chloral hydrate solution: add 0.3g of yam powder to 2ml of chloral hydrate solution with a mass concentration of 40% to make a mixture;

[0033] (3) Heat and permeabilize the mixture: put the mixture in a container, put it in the heating tank of the closed heating zone of the heating equipment or in the open heating zone, start the heating equipment, preheat to 110°C first, and then raise the temperature to 135°C ℃, heating for 8min to realize the permeabilization of the mixture;

[0034] (4) Preparation after permeabilization: drop 0.1ml of the permeabilized mixture in step (3) on the glass slide (25.4×76.2mm, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a chloral hydrate permeabilization flaking method in microscopic identification of TC, which can effectively solve the problem of easy control, high efficiency and safety of chloral hydrate permeabilization. The method comprises the following steps: adding water in chloral hydrate for dissolving to obtain a chloral hydrate solution with mass concentration of 30-50%; adding 0.1-0.5g of traditional Chinese medicinal material powder into 2ml of chloral hydrate solution with mass concentration of 30-50% to prepare a mixture; filling the mixture into a container, preheating to 100-110 DEG C, then heating to 125-135 DEG C for 4-8 minutes, realizing permeabilization of the mixture; dropping the 1-2 drops of permeabilized mixture on a slide glass, adding 1-2 drops of dilute glycerin, uniformly mixing the traditional Chinese medicinal material powder on the slide glass, covering a coverslip, using an absorbent paper to remove overflowed liquid, and keeping the clean coverslip and slide glass. The flaking method can be effectively used for chloral hydrate permeabilization, and has the advantages of energy saving, safety, high efficiency, controllable performance, wide application and environmental protection, is especially suitable for teaching and scientific research, has strong utility value, and is great innovation of chloral hydrate permeabilization flaking method.

Description

technical field [0001] The invention relates to the quality detection of traditional Chinese medicines, in particular to a method for permeabilizing and preparing tablets of chloral hydrate in traditional Chinese medicines for microscopic identification. Background technique [0002] Permeabilization of aldehyde hydrate into tablets is a common technical operation in the identification methods of traditional Chinese medicines, and the quality of the preparation directly affects the identification. However, with the development needs of teaching and scientific research, the shortcomings of this traditional permeabilization method gradually emerged. In addition to the danger of open flames in the use of alcohol lamps in the test, uneven heating and generation of Bubbles, scorched powder, etc., greatly affect the accuracy and efficiency of the experiment. [0003] The chloral hydrate permeabilization method is the most commonly used material processing method in the microscopi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 刘孟奇扶垭东高鹏王小巧陈随清
Owner HENAN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap