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Chicken muscle fiber-type property-related gene molecule marker and use thereof

A muscle fiber type and molecular marker technology, applied in the direction of microbial determination/inspection, DNA/RNA fragments, recombinant DNA technology, etc., to achieve low-cost results

Inactive Publication Date: 2015-07-08
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few reports about the PGC-1α gene in chicken muscle fiber types

Method used

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  • Chicken muscle fiber-type property-related gene molecule marker and use thereof
  • Chicken muscle fiber-type property-related gene molecule marker and use thereof
  • Chicken muscle fiber-type property-related gene molecule marker and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Select Chinese local high-quality chicken breed Qingyuan Maji and foreign introduced breed recessive white-feather broiler as examples of the present invention. The specific steps of applying the present invention are as follows:

[0053] Randomly select 200 hens of Qingyuan Ma chicken (17 weeks old) and recessive white-feather broiler (7 weeks old) at the market age. Blood is collected from the wing vein and placed in a 2ml EP tube containing anticoagulant for genomic DNA Extraction.

[0054] PCR amplification of the nucleotide sequence shown in SEQ ID NO. 1 (SEQ ID NO. 1 in the sequence listing is a nucleotide sequence containing the G646A mutation site of PGC-1α gene exon, with a length of 202 bp. There is a G97-A97 base mutation at 97bp.) The sequence information of the primer pair used is:

[0055] Upstream primer: 5'-TTCCATCGCCAAAATAAAAG-3' (shown in SEQ NO. 2),

[0056] Downstream primer: 5'-GGAGTTGCTGTAGCCAAAAT-3' (shown in SEQ NO. 3).

[0057] PCR amplification of the ...

Embodiment 2

[0083] In order to further determine the correlation between the genetic markers of the present invention and chicken muscle fiber type traits, the PCR-LDR rapid typing method established in Example 1 was used to select Qingyuan Ma chicken female and male hybrid F1 generations with both genotypes AA and Recessive white-feather broilers with AA genotypes were each crossed with 200 female and male F1 generations (marketing age). The fiber type ratio of the lateral gastrocnemius head muscle was determined. The results are shown in Table 3.

[0084] Table 3 Proportion of fiber types of the lateral gastrocnemius head in the F1 generation of the two chicken breeds

[0085]

[0086] It can be seen from Table 3 that the F1 generation of the two breeds of chickens has the highest proportion of slow muscle fibers. Compared with the AA genotype data in Table 2, the proportion of slow muscle fibers and fast red muscle fibers of the two breeds of chickens have increased after breeding. The prop...

Embodiment 3

[0087] Example 3: Application of the molecular genetic markers of the present invention in other broilers

[0088] Randomly select 200 hens each of Guangxi Sanhuang chicken (17 weeks old), Beijing Youchi (16 weeks old), Wenchang chicken (16 weeks old) and Anka chicken (7 weeks old) at the market age. The established PCR-LDR rapid typing method was used for genotyping. The genotype distribution results of the polymorphic loci of the four chicken breeds are shown in Table 4.

[0089] Table 4 Genotype distribution of polymorphic loci in four chicken breeds

[0090]

[0091] It can be seen from Table 4 that the frequency of allele G is higher in the four chicken breeds.

[0092] Use the general linear model of SAS9.0 software to analyze the association between the genotype and the percentage of chicken muscle fiber type, the model is Y ij =μ+B i +G j +e ij , Y ij Is the character performance value, μ is the overall mean, B i Is the variety effect, G j Is the genotype effect, e ij It i...

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Abstract

The invention discloses a chicken muscle fiber-type property-related gene molecule marker and a use thereof. A 646th nucleotide G-to-A mutational site of a PGC-1 alpha gene exon is used as a molecule marker, the molecule marker has a nucleotide sequence shown in the formula of SEQ ID NO. 1, and at 97th bp of the nucleotide sequence shown in the formula of SEQ ID NO. 1, G97-A97 base mutation is produced. The molecule marker provides a fast, simple and low cost gene analysis method for breeding of chicken with chicken muscle fiber-type properties, is conducive to screening of chicken with high slow muscle fiber content and good meat quality and can guide molecule marker-assistant breeding of chicken with chicken muscle fiber-type properties.

Description

Technical field [0001] The invention relates to the technical field of molecular biology, in particular to molecular markers of genes related to chicken muscle fiber type traits and their applications. Background technique [0002] With the continuous improvement of living standards and changes in consumption concepts, people have higher and higher requirements for the quality and taste of chicken, and the demand for high-quality chicken is increasing. High-quality chicken mainly emphasizes meat quality, and muscle fiber is the basic unit that constitutes muscle tissue. A large number of studies have shown that muscle fiber type is an important factor that directly affects muscle quality. Meat quality with more slow muscle fibers is relatively better, so cultivate slow muscle fiber content Many high-quality chicken breeds have become one of the goals of today's poultry breeders. [0003] With the rapid development of molecular quantitative genetics and molecular biology technology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 李慧芳束婧婷单艳菊徐文娟宋卫涛章明刘宏祥陶志云姬改革
Owner JIANGSU INST OF POULTRY SCI
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