Method for continuously preparing fructo-oligosaccharide by using immobilized enzyme microreactor

A microreactor and fructooligosaccharide technology, which is applied in the field of biocatalysis, can solve the problems of uncontinuous production and low reaction intensity, and achieve the effects of expanding production capacity, improving reaction efficiency and good industrialization prospects.

Active Publication Date: 2015-07-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a method for continuously preparing fructo-oligosaccharides using immobilized enzyme microreactors to solve the problems of low reaction intensity of synthetic fructo-oligosaccharides existing in the prior art and inability to produce continuously. Realize the orderly and controllable production mode of fructooligosaccharide

Method used

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  • Method for continuously preparing fructo-oligosaccharide by using immobilized enzyme microreactor
  • Method for continuously preparing fructo-oligosaccharide by using immobilized enzyme microreactor
  • Method for continuously preparing fructo-oligosaccharide by using immobilized enzyme microreactor

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Experimental program
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Effect test

Embodiment 1

[0025] Depend on figure 1 Shown, the microreactor of the present invention is made up of cover plate 1 and reaction plate 2, contains removable microchannel chip 3 in the groove of reaction plate center, by figure 2 It can be seen that the microchannel chip 3 is engraved with a channel tightly meandering, the upper surface of the channel is open, the lower surface and sides of the channel are closed, the total length of the channel is 50-200mm, and the cut surface of the channel is a trapezoidal structure with a wide top and a narrow bottom ( image 3 ), the long side of the trapezoid is 0.5-1.5mm, the short side is 0.1-0.4mm, and the height is 0.3-1.0mm, the four corners of the trapezoid are rounded, and the rounded diameter is 0.1mm; A fixing clip is provided between the cover plate 1 and the reaction plate 2 of the reactor to prevent side leakage of the solution, wherein the material of the cover plate is glass or stainless steel, and the material of the reaction plate is ...

Embodiment 2

[0027] 1g TiO 2 Disperse in 50ml of toluene, add 5ml of 3-mercaptopropyltrimethoxysilane, and reflux in an oil bath at 110°C for 8h. The sample was suction filtered and washed several times with toluene; the filtered sample was placed in a Soxhlet extractor and extracted with ethanol at reflux temperature for 24 hours to remove unreacted coupling agent; after suction filtration, the sample was taken out at 60 After drying at ℃ for 24h, SH-TiO can be obtained 2 .

[0028] Apply polydimethylsiloxane sol to the inside of the cover plate, and then use a sieve plate with an aperture of 800 mesh to filter the SH-TiO 2The powder is evenly distributed on the polydimethylsiloxane sol, and baked in an oven at 60-80°C for 100-200min to solidify the sol.

[0029] Inject the inulin endoenzyme solution into the removable microchannel chip 3, control the flow rate at 1-100 μL / min, and keep the retention time at 1-5 hours, rinse the excess enzyme solution with ultrapure water to realize th...

Embodiment 3

[0032] The device structure is as in Example 1, 5ml of an aqueous solution with an inulin content of 5wt%, and sulfuric acid to adjust the pH to 4.6, and the reaction system is continuously pumped into the microreactor by a syringe pump at a flow rate of 100 μL / min. The flow channel in the microreactor is a trapezoidal structure with a wide top and a narrow bottom. The long side is 0.5mm, the short side is 0.1mm, the height is 0.5mm, and the channel length is 50mm. 20mg, the reaction temperature is controlled at 55°C, and the retention time of the reaction feed liquid circulating and continuously flowing in the reactor is 4h. The yield of fructo-oligosaccharide prepared by enzymatic degradation was 99.5% detected by HPLC.

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Abstract

The invention discloses a method for continuously preparing fructo-oligosaccharide by using an immobilized enzyme microreactor. The method comprises the steps of fixing endo-inulinase in the microreactor, injecting a substrate, namely an inulin aqueous solution into the microreactor, wherein the flow rate of the substrate is 0.1-1mL / min, and the reaction temperature is 55-65 DEG C, the retention time of circulation and continuous flow of reaction feed liquid in the microreactor is 2-6 hours, and degrading the inulin solution to the fructo-oligosaccharide. The method has the advantages that the microreactor is used for enzymatic hydrolysis of inulin to generate the fructo-oligosaccharide, the reaction efficiency is greatly improved, and the energy consumption is reduced; meanwhile, the content of nystose in the product can be remarkably increased by controlling operating conditions, and the effects of the fructo-oligosaccharide on prevention and treatment of cardiovascular diseases are improved. A micro-reaction chip of the microreactor can be replaced, the microreactor is simple in structure, as long as the number of the microreactor is increased, the production capacity can be expanded in an equal proportion manner, and therefore, the method has good industrialization prospect.

Description

technical field [0001] The invention relates to the field of biocatalysis, in particular to a method for continuously preparing fructo-oligosaccharides by using a microreactor to enzymatically hydrolyze inulin. Background technique [0002] Fructo oligosaccharide (FOS) is a natural active substance. Fructo oligosaccharides, also known as fructooligosaccharides, are composed of 2 to 9 fructosyl groups and fructosyl groups in sucrose through β (2-1) glycosidic bonds. The sweetness of the fructose polysaccharide mixture produced by combining is 0.3-0.6 times that of sucrose. It maintains the pure sweetness of sucrose, and is refreshing than sucrose; it is a new sweetener with health functions such as regulating intestinal flora, proliferating bifidobacteria, promoting calcium absorption, regulating blood lipids, immune regulation, and anti-caries. Fructose-oligosaccharides are known as the most potential new generation of additives after the era of antibiotics - bio-promoting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 韦策蒋羽佳张庆文李干禄姜岷
Owner NANJING UNIV OF TECH
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