Microfluidic device for automatic extraction of nucleic acid

Abstract

The invention provides a microfluidic device for automatic extraction of nucleic acid. The microfluidic device comprises a microfluidic chip and a driving device. Before an experiment, a sample, a lysis solution, magnetic beads and the like are injected into a lysis chamber of the microfluidic chip respectively by virtue of an injection hole; a cleaning solution is injected into three washing chambers; an eluent is injected into an elution chamber; then the microfluidic chip is put into a chip tray, and tight fit is formed; a step motor drives the rotation of the chip tray and the positioning control of a groove type optocoupler; the adsorption effect of upper and lower magnets on the magnetic beads is utilized to ensure that the microfluidic chip rotates back and forth in a small range to promote the reaction between the sample and the lysis solution, between the magnetic beads and the cleaning solution and between the cleaning solution and the eluent, and by virtue of a baffle plate at the inlet of each reaction chamber, the transfer between the magnetic beads and to-be-extracted nucleic acid can be performed among the chambers, thereby finishing multiple reaction steps of cell lysis, nucleic acid purification and nucleic acid elution to obtain a purified nucleic acid template and realize automatic extraction of nucleic acid.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a microfluidic device for automatically completing the nucleic acid extraction process. Background technique [0002] Due to its unique technical characteristics and advantages, microfluidic chip technology has attracted close attention from researchers all over the world, and is gradually making various breakthroughs. In principle, a microfluidic chip can transplant basic operating units such as sample preparation, mixing, reaction, separation, detection, cell culture, sorting, and lysis involved in the fields of chemistry or biology to a small chip. Or integrate multiple functional modules into a unified chip, and run the controllable fluid through the microchannel network of the entire chip to build biological and chemical microreactors or microsystems. For the field of biomedical diagnosis, its significant advantages lie in: through the automatic and streamlined working m...

AI Technical Summary

Problems solved by technology

Existing nucleic acid extraction methods often include multiple complex biological reaction steps, whether done by hand or relying on auxiliary equipment, there are disadvantages such as low efficiency and error-prone

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