Method for preparing anthocyanin standard substance

An anthocyanin and standard product technology, applied in the field of phytochemistry, can solve the problems of separation and purification, many non-anthocyanin impurities, unclear composition of anthocyanin components, etc., and achieves good stability and high purity. Effect

Inactive Publication Date: 2015-07-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology for extracting and separating anthocyanins from Camellia safflower generally only obtains anthocyanin-containing stock solution products or crude extracts, and cannot differentiate, separate and purify different types of anthocyanins. Among the extracts, non- There are too many anthocyanin impurities, the total anthocyanin content is low, and the composition of various anthocyanin components is unclear. Although it can be used as a food natural pigment, it is difficult to give full play to the inherent physiological functions of anthocyanin. , cannot be directly applied in the fields of medicine, bioengineering, chemical industry, etc.

Method used

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  • Method for preparing anthocyanin standard substance
  • Method for preparing anthocyanin standard substance
  • Method for preparing anthocyanin standard substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1Cy3

[0050] The separation and purification of embodiment 1Cy3GX type anthocyanins

[0051] A method for preparing anthocyanin standard substance, the method for preparing this type of anthocyanin standard substance is carried out according to the following steps:

[0052] 1) Petal collection of safflower camellia

[0053] Use wild species of Camellia yunnanensis, horticultural varieties or hybrid varieties containing the blood of Camellia yunnanensis as raw materials for preparation. During the flowering period of camellia, collect red and brightly colored flowers, remove impurities other than stamens and petals, and use them to extract anthocyanins , in the process of collecting and selecting petals, try to prevent the petals from being damaged and browned;

[0054] 2) Preparation of anthocyanin stock solution

[0055] ①Put the collected petals into the container, compact them slightly with your hands, then continue to add petals, and add them to a place 2-3cm below the mouth o...

Embodiment 2

[0072] The separation and purification of embodiment 2Cy3G5G type anthocyanins

[0073] A method for preparing anthocyanin standard substance, the method for preparing this type of anthocyanin standard substance is carried out according to the following steps:

[0074] 1) Petal collection of safflower camellia

[0075] Use Nujiang camellia or camellia gardening varieties with blood background of Nujiang camellia as raw materials for preparation. During the flowering period of camellia, collect red and brightly colored flowers, remove impurities other than stamens and petals, and extract anthocyanins from the petals. During the collection and selection process, try to prevent the petals from being damaged and browned;

[0076] 2) Preparation of anthocyanin stock solution

[0077] ①Put the collected petals into the container, compact them slightly with your hands, then continue to add petals, and add them to a place 2-3cm below the mouth of the container;

[0078] ② Mix aceti...

Embodiment 3

[0089] The separation and purification of embodiment 3Cy3G type anthocyanins

[0090] Camellia, or horticultural varieties of camellia are used as raw materials for preparation, and the specific separation and purification methods are consistent with the separation and purification methods and steps of Cy3GX type anthocyanins in Example 1.

[0091] This preparation method can mainly obtain Cy3G(6), Cy3Ga(4), Cy3GZpC(12), Cy3GaEpC(15), Cy3GECaf(11) and Cy3GEpC(19) and other anthocyanin standard products.

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Abstract

The invention discloses a method for preparing an anthocyanin standard substance and belongs to the field of phytochemistry. The method comprises the following steps of carrying out extraction on red fresh petals (or dry petals) of camellia by an acidic ethanol aqueous solution at a room temperature for 12-14h, filtering the extract, then carrying out pressure reduction condensation, and based on acidic edible alcohol and water as mobile phases, carrying out column chromatography treatment orderly by macroporous resin, gel and ODS as filling materials so that 19 anthocyanin monomers are separated. The method can extract and separate more anthocyanin monomers from petals of other plants. Through combination with a crystallization technology, the method has better effects of separating water-insoluble polyphenol monomers from leaves or petals. The anthocyanin monomer prepared by the method has high purity and good stability, can be used as a standard substance and can be used in fields of medicine, health products, cosmetics, foods, bioengineering and chemical engineering.

Description

technical field [0001] The invention belongs to the field of phytochemistry, and in particular relates to a method for preparing standard anthocyanins. Background technique [0002] Anthocyanin is a water-soluble pigment formed by condensation of anthocyanidin and sugar through glycosidic bonds. It exists in the vacuole of plant epidermal cells and appears orange, red to blue. One of the important pigments for the color of organs such as fruits, seeds and leaves. In the petals, anthocyanins gradually accumulate along with the development of the flower, and in the late stage of petal development, anthocyanins are produced in large quantities. Nutrient deficiency, ultraviolet radiation, low temperature, frost, drought and other environmental factors can induce the synthesis and accumulation of anthocyanins. Anthocyanins are easy to absorb visible light and ultraviolet light, effectively eliminate active oxygen free radicals produced by the photosynthetic electron transfer sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08C09B61/00
Inventor 李建宾希从芳薛英利赵庆师李纪元
Owner YUNNAN AGRICULTURAL UNIVERSITY
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