Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for converting ovarian granular cells into multipotential stem cells

A technology of pluripotent stem cells and granule cells, applied in the fields of biology and new medicine, can solve problems such as carcinogenesis and iPS cell safety, and achieve the effect of improving the induction efficiency

Inactive Publication Date: 2015-07-01
NANKAI UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional means of inducing iPS cells is to introduce four reprogramming factors, Oct4, Sox2, Klf4, and c-Myc (also known as Yamanaka factors), into fibroblasts through retroviral vectors to induce their reprogramming. The iPS cells obtained by this method have safety problems, especially the potential carcinogenic effects of these factors and the integration of exogenous factors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for converting ovarian granular cells into multipotential stem cells
  • Method for converting ovarian granular cells into multipotential stem cells
  • Method for converting ovarian granular cells into multipotential stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The method of converting ovarian granulosa cells into pluripotent stem cells can be widely applied to human, mouse, pig and other species. Our method has been successfully applied to mice and pigs, and the success in mice and pigs also validates our method.

[0019] Ovarian granulosa cells are isolated and transformed into pluripotent stem cells by reprogramming factors or small molecular compounds after adherence. Identification of the obtained pluripotent stem cells, including activation of endogenous pluripotency genes and silencing of exogenous genes, expression of pluripotency markers, in vivo differentiation (teratoma formation) and in vitro differentiation (embryoid body differentiation) The ability to form chimeras and germline transfer. The obtained pluripotent stem cells can be widely used in human medical clinics, transgenic applications, and the like.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method of converting ovarian granular cells serving as precursor cells into multipotential stem cells. After the ovarian granular cells are separated and attached, measures such as reprogramming factors or small molecule compounds are adopted to convert the ovarian granular cells into the multipotential stem cells. In the experiment of mice, an operator successfully converts the ovarian granular cells of the mice into the true multipotential stem cells efficiently. In the experiment of pigs, the operator also obtains a good multipotential stem cell system in a high-efficiency manner. The ovarian granular cells have the advantages of wide source, high reprogramming efficiency and the like, and the obtained multipotential stem cells made from ovarian granular cells have the higher safety and great potential application to the clinical medicines of people. The method is efficient, economical, good in safety and wide in application prospect.

Description

technical field [0001] The invention relates to converting ovarian granulosa cells into pluripotent stem cells, and belongs to the technical field of biology and new medicine. Background technique [0002] In the follicle, the granulosa cells are the cells surrounding the oocyte, which have certain pluripotency. There are two types of granulosa cells: those that surround the oocyte, called cumulus cells, and those that surround the lumen, called parietal granulosa cells, and both have been used with high efficiency in animal cloning. Nonetheless, granulosa cells are often discarded as a by-product of in vitro fertilization or the livestock industry and do not receive the attention they deserve. [0003] Pluripotent stem cells (pluripotent stem cells) refer to a type of cells that have the potential to form all types of cells in humans and animals and can continuously renew themselves. Due to the multi-directional differentiation potential and self-renewal characteristics o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10
Inventor 毛剑张倩叶孝颖刘锴刘林
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com