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Method for converting ovarian granular cells into multipotential stem cells

A technology of pluripotent stem cells and granule cells, applied in the fields of biology and new medicine, can solve problems such as carcinogenesis and iPS cell safety, and achieve the effect of improving the induction efficiency

Inactive Publication Date: 2015-07-01
NANKAI UNIV
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Problems solved by technology

The traditional means of inducing iPS cells is to introduce four reprogramming factors, Oct4, Sox2, Klf4, and c-Myc (also known as Yamanaka factors), into fibroblasts through retroviral vectors to induce their reprogramming. The iPS cells obtained by this method have safety problems, especially the potential carcinogenic effects of these factors and the integration of exogenous factors

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  • Method for converting ovarian granular cells into multipotential stem cells
  • Method for converting ovarian granular cells into multipotential stem cells
  • Method for converting ovarian granular cells into multipotential stem cells

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Embodiment Construction

[0018] The method of converting ovarian granulosa cells into pluripotent stem cells can be widely applied to human, mouse, pig and other species. Our method has been successfully applied to mice and pigs, and the success in mice and pigs also validates our method.

[0019] Ovarian granulosa cells are isolated and transformed into pluripotent stem cells by reprogramming factors or small molecular compounds after adherence. Identification of the obtained pluripotent stem cells, including activation of endogenous pluripotency genes and silencing of exogenous genes, expression of pluripotency markers, in vivo differentiation (teratoma formation) and in vitro differentiation (embryoid body differentiation) The ability to form chimeras and germline transfer. The obtained pluripotent stem cells can be widely used in human medical clinics, transgenic applications, and the like.

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Abstract

The invention discloses a method of converting ovarian granular cells serving as precursor cells into multipotential stem cells. After the ovarian granular cells are separated and attached, measures such as reprogramming factors or small molecule compounds are adopted to convert the ovarian granular cells into the multipotential stem cells. In the experiment of mice, an operator successfully converts the ovarian granular cells of the mice into the true multipotential stem cells efficiently. In the experiment of pigs, the operator also obtains a good multipotential stem cell system in a high-efficiency manner. The ovarian granular cells have the advantages of wide source, high reprogramming efficiency and the like, and the obtained multipotential stem cells made from ovarian granular cells have the higher safety and great potential application to the clinical medicines of people. The method is efficient, economical, good in safety and wide in application prospect.

Description

technical field [0001] The invention relates to converting ovarian granulosa cells into pluripotent stem cells, and belongs to the technical field of biology and new medicine. Background technique [0002] In the follicle, the granulosa cells are the cells surrounding the oocyte, which have certain pluripotency. There are two types of granulosa cells: those that surround the oocyte, called cumulus cells, and those that surround the lumen, called parietal granulosa cells, and both have been used with high efficiency in animal cloning. Nonetheless, granulosa cells are often discarded as a by-product of in vitro fertilization or the livestock industry and do not receive the attention they deserve. [0003] Pluripotent stem cells (pluripotent stem cells) refer to a type of cells that have the potential to form all types of cells in humans and animals and can continuously renew themselves. Due to the multi-directional differentiation potential and self-renewal characteristics o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10
Inventor 毛剑张倩叶孝颖刘锴刘林
Owner NANKAI UNIV
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