A high-throughput dna sequencing chip

A DNA sequencing, high-throughput technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of low accuracy and low sequencing throughput, and achieve high sensitivity and simple detection , the effect of large effective area

Active Publication Date: 2016-04-20
BEIJING ZHONGKEZIXIN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microfluidic gene chip still has the disadvantages of low sequencing throughput and low accuracy, so it is necessary to prepare a new type of gene chip to meet the needs of practical applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A high-throughput DNA sequencing chip, the chip is 49 mm long, 48 mm wide, and 2 mm thick. The chip is equipped with 680,000 to 690,000 reaction pools, the diameter of which is 30 μm, and a top cover on the top of the reaction pool. The material is optical fiber, and the effective area of ​​the chip is 27.6x27.6mm 2 . Chips were fabricated using the Infinium chip fabrication method.

Embodiment 2

[0014] A high-throughput DNA sequencing chip. The chip is 49mm long, 48mm wide, and 2mm thick. The chip is equipped with 690,000 to 700,000 reaction pools. The diameter of the reaction pool is 29 μm. There is an upper cover on the top of the reaction pool. The material is quartz, and the effective area of ​​the chip is 27.6x27.6mm 2 . Chips were fabricated using the Infinium chip fabrication method.

Embodiment 3

[0016] A high-throughput DNA sequencing chip with a length of 49mm, a width of 48mm, and a thickness of 2mm. The chip is equipped with 700,000 to 710,000 reaction pools, the diameter of which is 28.5 μm, and an upper cover on the top of the reaction pool. The material is optical fiber, and the effective area of ​​the chip is 27.6x27.6mm 2 . Chips were fabricated using the Infinium chip fabrication method.

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Abstract

The invention belongs to the field of biomedical engineering equipment manufacturing technology and relevant devices, and particularly relates to a high-flux DNA (Deoxyribonucleic Acid) sequencing chip. The chip is provided with reaction tanks; an upper cover is arranged above the reaction tanks; the chip is made of optical fiber or quartz; 680,000-720,000 reaction tanks are arranged on the chip; the diameters of the reaction tanks are 28-30 microns; the chip is 49 millimeters in length, 48 millimeters in width and 2 millimeters in thickness. The chip is manufactured by using an Infinium manufacturing technology. By adopting the high-flux sequencing chip, the sequencing accuracy and efficiency are ensured.

Description

technical field [0001] The invention belongs to the field of biomedical engineering equipment manufacturing technology and related devices, and in particular relates to a high-throughput DNA sequencing chip. Background technique [0002] The completion of the Human Genome Project has had a huge impact on contemporary biological and medical research. People can understand the differences of life phenomena, the law of disease occurrence and development, and the interaction between drugs and living organisms at the genetic level. As far as gene sequence analysis is concerned, the focus of the post-genome era has shifted from the determination of the whole genome sequence of a single species to the comparison of individual genetic differences and inter-species genetic differences of a certain species at the level of genome DNA sequence. In terms of basic research, it studies the genetic laws of disease genes and clones disease-causing genes; in terms of applications, it directl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00
Inventor 陈哲张睿王者馥王绪敏殷金龙任鲁风
Owner BEIJING ZHONGKEZIXIN TECH
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