Method for extracting and separating sapindoside B from plant natural saponine soapberries

Abstract

The invention discloses a method for extracting and separating total saponins of soapberries and sapindoside B from pericarps of soapberries. The method comprises the following steps: drying and crushing peel of soapberries, extracting by using a solvent, filtering and concentrating to obtain an extract; after mixing the extract, dissolving with water, absorbing by using macroporous resin, eluting by a solution and separating to obtain the component of the total saponins of soapberries; and then tracking and determining through TLC and HPLC, carrying out column chromatography on a silica gel on the eluted part containing the sapindoside B and then carrying out gel column chromatography to finally obtain the sapindoside B. By virtue of macroporous resin separation, column chromatography on silica gel and gel column chromatography matched with technologies such as HPLC and TLC to track and guide separation and the like, a sapindoside B product with the purity of 90% or above is separated from the soapberries. The method is simple and controllable to operate, the obtained product is low in impurity content and high in purity, and the prepared sapindoside B can be used as a standard product reaching the quality standard of sapindoside, thereby laying a foundation for quality control of soapberries and further more reasonable development and utilization.

Description

technical field [0001] The invention relates to a method for extracting and separating plant natural saponins, in particular to a method for extracting and separating Sapindus saponin B from Sapindus chinensis. Background technique [0002] Sapindus mukorossi (Latin scientific name: Sapindus mukorossi Gaertn.), Sapindus mukorossi Gaertn., Sapindus family Sapindus, perennial deciduous trees, native to the south of the Yangtze River Basin in my country, Indochina Peninsula, India and Japan. According to legend, the wooden stick made of the wood of the Wuzai tree can exorcise demons and kill ghosts, so it is called Wuzai. The Latin scientific name Sapindus is the abbreviation of soap indicus, which means "soap of India", because its thick fleshy peel contains saponin, as long as it is rubbed with water, it will produce foam, which can be used for cleaning. It is one of the main cleaning agents in ancient times. one. In addition, in addition to its cleansing effect, Sapindus c...

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Problems solved by technology

[0004] Chinese patent application CN 1097764 discloses a method for extracting a saponin-containing substance solution from the shell of Sagittarius oleifera, which includes processes such as drying, shelling, shell crushing, extraction (soaking), sedimentation, filtration, rectification, etc. , finally can obtain mainly containing sapin, also containing amino acid, vitamin, and total effective substance amount is the aqueous solution product of 15-28% (wt%), but in the Sapindia chinensis that this method extracts is mixture, can not be used for Sapindia sapindus saponin B separate extraction of

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