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Application of adiponectin in preparing medicine for treating fracture

A technology for the treatment of fractures and adiponectin, which can be used in drug combinations, bone diseases, pharmaceutical formulations, etc., and can solve the problems of optimal dosage and no relevant research on the way of administration, so as to accelerate callus formation and achieve good therapeutic effects. , the effect of shortening the time of bone healing

Active Publication Date: 2015-07-01
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, although the prior art has determined that adiponectin can affect bone metabolism, there is no relevant research on the effect of adiponectin on traumatic fracture repair, callus strength, etc., its optimal dosage, and administration method. This is unknown to those skilled in the art

Method used

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  • Application of adiponectin in preparing medicine for treating fracture
  • Application of adiponectin in preparing medicine for treating fracture
  • Application of adiponectin in preparing medicine for treating fracture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Cell Intervention Experiment

[0036] Extraction of rat osteoblasts: take the cancellous bone of the ilium of the rat surgically and cut it into 2mm×1mm×1mm fragments, wash and shake with phosphate buffered saline (PBS) for 3 times, add 1g / L type IV Shake and digest collagenase at 37°C. When the cancellous bone turns white and takes on a honeycomb shape, add phenol red-free MEM containing 0.2 volume fraction of fetal bovine serum to terminate the digestion, and use MEM containing 0.1 volume fraction of fetal bovine serum Wash 3 times, when the washing solution does not contain cells, paste the bone slices on the 25cm 2 Add MEM containing 0.15 volume fraction of fetal bovine serum to the wall of the culture flask, which contains 50 mg / L ascorbic acid, 50 U / mL penicillin, and 50 mg / L gentamicin. 2 cultivated under conditions. Change the medium two days later, and then change the medium once every 3 days. After about 15 days of culture, cells can be seen to swi...

Embodiment 2

[0054] Embodiment 2 common animal intervention experiment

[0055] 2 μg / g adiponectin was injected into the tail vein of common C57 mice (male, 4 weeks old, 20-25g, purchased from the animal room of PLA General Hospital) for 4 weeks, 8 weeks, and 12 weeks, with 6 mice in each group at different time periods. Micro-CT (MicroCT) measures bone cortical thickness and cancellous bone content, and the results show that adiponectin can improve bone structure. The result is as image 3 shown.

[0056] Depend on image 3 A It can be seen that after 2 μg / g adiponectin injection for 4 weeks, 8 weeks, and 12 weeks, the volume of cancellous bone in mice was higher than that of the control group, and the difference between 8 weeks and 12 weeks was significant, and the content of cancellous bone reached peak.

[0057] Depend on image 3 B It can be seen that after 2 μg / g adiponectin injection for 4 weeks, 8 weeks, and 12 weeks, the cortical bone thickness of the mice was higher than tha...

Embodiment 3

[0058] Example 3 Adiponectin Intervention of Linear Fracture Animals

[0059] Femur linear fracture model: C57 mice (male, 4 weeks old, 20-25g, purchased from the animal room of PLA General Hospital) were randomly divided into groups, 6 mice in each group, the mice were anesthetized and hair removed, and a longitudinal incision was made from the lower end of the femur , expose the lower end of the femur, penetrate a 1ml syringe needle, enter the quadriceps muscle space, expose the femur, cut the femur with scissors, form a linear fracture, and suture the wound.

[0060] Local injection of adiponectin: Inject 1-1.5 ml of physiological saline containing 10 μg, 20 μg, and 50 μg adiponectin into the left bone stump of the above-mentioned linear femur fracture mouse model, as the experimental group. The right bone stump was not injected with adiponectin, and served as the control group, which was injected with the same amount of normal saline. Another group administered with bone ...

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Abstract

The invention provides an application of adiponectin in preparing a medicine for treating fracture. Tests prove that the adiponectin can significantly accelerate porosis, enhance the strength of callus, increase fracture healing speed and shorten the time of bone healing, so as to promote bone healing; the adiponectin has a good effect on treating comminuted fracture, linear fracture, bone defect and the like, and especially, the adiponectin has a remarkable promoting effect on femoral fracture which is slow in healing; therefore, a new method is offered to the clinical treatment of fracture.

Description

technical field [0001] The present invention relates to the new application of adiponectin in pharmacy, more specifically, relates to the application of adiponectin in the preparation of medicine for treating fractures. Background technique [0002] Limb injuries account for a very high proportion of war injuries, among which fractures account for about 60% of limb injuries caused by war injuries. In the usual industrial and agricultural production, transportation, sports and military training, fractures are also common accidents. In urban trauma centers, about 45.5% of hospitalized patients had fractures, among which femoral shaft fractures were the most common limb fractures. [0003] Fracture healing time varies depending on the site, and usually the repair time of lower extremity fractures is relatively long. Femoral shaft fractures generally take 8 to 12 weeks, while femoral neck fractures take the longest recovery time, which takes 12 to 24 weeks. The healing time f...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61P19/08
Inventor 龚燕平李春霖万丽娟祖源王鑫李丙岩
Owner GENERAL HOSPITAL OF PLA
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