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Preparation method and application of electrochemical sensing element based on immunogold

A sensing element and immune gold technology, applied in the field of electrochemistry, can solve the problems of complex processing, expensive detection equipment, difficulty in satisfying rapid detection and on-site detection of batch samples, and achieve the effect of large surface area

Inactive Publication Date: 2015-06-24
CHINA JILIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection equipment of these methods is expensive, the sample pretreatment is complicated, and it also requires high-level professionals to operate, which is difficult to meet the requirements of rapid detection and on-site detection of batch samples.

Method used

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  • Preparation method and application of electrochemical sensing element based on immunogold
  • Preparation method and application of electrochemical sensing element based on immunogold
  • Preparation method and application of electrochemical sensing element based on immunogold

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Embodiment 1

[0042] 1.1 Reagents

[0043] Metronidazole former medicine (Metronidazole), purity 99%, 25g, J&K Chemical Company; Tinidazole, Dimetonidazole, Ronidazole, Ipronidazole, N, N'-dimethylformamide (DMF ) and N-hydroxysuccinimide (NHS), both analytically pure, Sigma Company; N, N'-dicyclohexylcarboimide (DCC) and carbodiimide hydrochloride (EDC), chemically pure , Sinopharm Chemical Reagent Co., Ltd.; copper nitrate and trimercaptopropionic acid (MPA) were purchased from Aladdin Company.

[0044] 1.2 Instruments

[0045] CHI400 series electrochemical quartz crystal microbalance (Shanghai Chenhua Instrument Co., Ltd.), 0.3 μm and 0.05 μm Al 2 o 3 Polishing and polishing instrument (Shanghai Chenhua Instrument Co., Ltd.), nitrogen purging instrument (Hangzhou Aosheng Instrument Co., Ltd.), Z50 Darwin nanometer particle size analyzer.

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Abstract

The invention discloses a preparation method of an electrochemical sensing element based on immunogold. A common nitroimidazole type structure hapten is prepared, and the nitroimidazole hapten is coupled with carrier proteins (BSA and OVA) by adopting an active ester method to prepare an immunogen and a coating antigen, wherein the mol ratio of coupling molecules is (11 to 1) and (10 to 1). A nitroimidazole antibody is simultaneously prepared and the valence is (1 to 100000), and immune nano gold is prepared; and a monomolecular film is formed on a gold electrode by using a monomolecular film self-assembling technology, and is connected with the immune nano gold through DCC and NHS to prepare the sensing element. The sensing element has the good responses on metronidazole, tinidazole and dimetridazole; the cross reaction rate is 90% or more; metronidazole, tinidazole and dimetridazole and metabolites of metronidazole, tinidazole and dimetridazole in a concentration range of 0.001-100mg / L can be determined; and the detection limit is about 0.0019mg / L and the recycling rate is about 90%.

Description

technical field [0001] The invention relates to the field of electrochemical technology, in particular to a preparation method and application of an immunogold-based electrochemical sensing element. Background technique [0002] Nitroimidazole antibiotics include metronidazole, tinidazole, demetonidazole, ronidazole, and ipronidazole, etc., which are commonly used in livestock and poultry feed for anti-protozoal infection and antibacterial drugs, and have growth-promoting effects. It can also be used in combination with other antibiotics to treat anaerobic infections. However, these drugs and some of their metabolites have carcinogenic, teratogenic, mutagenic and genotoxic effects on mammals, and are listed as prohibited drugs by many countries. In the European Union, Lonida, Dimenidazole and Metronidazole were banned from being used in food animals in 1993, 1995 and 1998 respectively. Eleven kinds of drugs were used, including Dimetonidazole and other nitroimidazoles. An...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N27/30
CPCG01N33/5438
Inventor 潘家荣帅瑞琦方豪冯依璠
Owner CHINA JILIANG UNIV
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