Method for preparing polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing

Abstract

The invention discloses a method for preparing a polyving akohol / nanosized silica composite hydrogel scaffold through 3D printing. The method comprises the steps that firstly, polyving akohol (PVA) resin with the mass concentration of 8-12% is added into deionized water to be stirred and dispersed, and is completely decomposed in a thermostatic waterbath of 90 DEG C to 95 DEG C to obtain a PVA solution; the obtained PVA solution is slowly added into inorganic nano silicon oxide (SiO2), and is evenly stirred to obtain mixed collosol with the thixotropic property, wherein the mass ratio of the nano SiO2 to the PVA is (0.5-1):1; then, a robot dispenser is utilized for extruding the mixing collosol, and through 3D printing forming, a collosol scaffold sample is obtained; and finally, the sample is placed in a freezing chamber at the temperature of minus 20 DEG C to minus 40 DEG C to be frozen for 20 hours to 22 hours, after the sample is taken out of the chamber, the sample is unfrozen and melted for 2 hours to 4 hours at the room temperature, and the hydrogel scaffold of a controllable fine structure is obtained. The method can conveniently and rapidly prepare the biological scaffold, and controllability of a support structure and inner pores is achieved, so that the method adapts to needs of different application occasions.

Description

technical field [0001] The present invention relates to a method for rapid prototyping of hydrogel biological scaffolds, more specifically, the present invention relates to a method for preparing polyvinyl alcohol / nano-silica composite hydrogel scaffolds by 3D printing, which belongs to biomedical polymer materials technology field. Background technique [0002] Human beings often cause damage to articular cartilage due to aging, accidents and other reasons, and cartilage, as a tissue with very weak regenerative ability, is a major problem in its repair. At present, the most clinically used method is to repair with biological materials, but this method will cause the body's rejection reaction. With the development of tissue engineering technology, using biological scaffolds as carriers, combined with cartilage seed cells and growth factors, to construct cartilage tissue through in vivo or in vitro culture to repair injured tissue is a very promising repair method. The biol...

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings in the preparation of PVA hydrogel bioscaffolds - the shape of the scaffold and the internal pores of the scaffold cannot be adjusted conveniently, and the fineness of the scaffold is not high enough.

And PVA hydrogel is applied to articular cartilage replacement material, its strength is still low

[0003] Chinese Patent Publication No. CN101544767A discloses a method for preparing a biocompatible high-strength three-dimensional interconnected porous PVA hydrogel. The method uses polyvinyl alcohol as a matrix material, utilizes surfactants and soluble solid particles to form pores, and uses Three-dimensional porous polyvinyl alcohol hydrogel was prepared by ultrasonic cleaning. Although this method can obtain a network structure of three-dimensional through holes, it cannot realize the controllable and adjustable scaffold structure and hole shape.

Chinese Patent Publication No. CN103120806A discloses a method for preparing a biocartilage scaffold based on PVA hydrogel. The method uses hydroxyapatite microspheres as the base material, and prints a cartilage molding mold through a 3D printer to form PVA containing bone cells. Hydrogel, although this method achieves zero pollution in the preparation of scaffolds and shortens the preparation cycle, but using molds to indirectly form PVA hydrogels cannot obtain bioscaffolds with complex structures

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