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Method for preparing feed by transforming various cellulose-containing by-products by using Rhodospira rubrum rubrum

A technology of Rhodospirillum rubrum and cellulose, applied in animal feed, animal feed, applications, etc., can solve the problems of unseen feed production reports, long production cycle, etc., to shorten the production cycle, increase value, and expand raw materials category effect

Active Publication Date: 2017-12-12
YIBIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the need to ferment under absolute anaerobic conditions, and the raw material is specific distiller's grains, and the entire production cycle is long (combined with Trichoderma viride step by step), there are many inconvenient factors
[0005] Rhodospirillum rubrum is a purple non-sulfur bacterium among photosynthetic bacteria. There has been a lot of research on its use in hydrogen production and sewage treatment, but there is no report on its solid-state fermentation for feed production.

Method used

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  • Method for preparing feed by transforming various cellulose-containing by-products by using Rhodospira rubrum rubrum
  • Method for preparing feed by transforming various cellulose-containing by-products by using Rhodospira rubrum rubrum
  • Method for preparing feed by transforming various cellulose-containing by-products by using Rhodospira rubrum rubrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method of producing feed by acid hydrolysis with discarded grains, primary neutralization, ammoniation, secondary neutralization, and fermentation of Rhodospira rubrum comprises the following steps:

[0025] (1) Raw material handling. The dregs with a moisture content of 9.9% after drying are pulverized into 40 mesh uniform particles;

[0026] (2) Acid hydrolysis. Mix 100 parts of pulverized grains with 100 parts of 1% sulfuric acid solution, heat up to 90° C. for 1 h, and the process needs to replenish water to the original volume;

[0027] (3) One time neutralization. Add 1.6 parts of CaCO to the above acid hydrolyzate 3 Neutralize and stir evenly;

[0028] (4) Amination. Add 400 parts of 0.2% ammonia water to the above-mentioned neutralization product, and keep it airtight and insulated at 90° C. for 1.5 h;

[0029] (5) Secondary neutralization. The amide is cooled to 35 °C, and the pH is adjusted to 7.0 with a 1% concentration of phosphoric acid solution;...

Embodiment 2

[0034] The method for preparing feed by acidolysis of sauce residue, primary neutralization, ammoniation, secondary neutralization, and fermentation of Rhodospirillum rubrum comprises the following steps:

[0035] (1) Raw material handling. Grinding the dried sauce residue with a moisture content of 10.2% into 60-mesh uniform particles;

[0036] (2) Acid hydrolysis. Mix 100 parts of pulverized sauce residue with 150 parts of 1% phosphoric acid solution evenly, heat up to 100°C and keep it warm for 1.5 h. During the process, water needs to be replenished to the original volume;

[0037] (3) One time neutralization. Add 2 parts of CaCO to the above acid hydrolyzate 3 , and stir evenly;

[0038] (4) Amination. Add 250 parts of 0.3% ammonia water to the above-mentioned neutralization product, and keep it airtight and insulated at 95° C. for 2 h;

[0039] (5) Secondary neutralization. The amide is cooled to 36 ° C, and the pH is adjusted to 7.0 with 1% sulfuric acid solution...

Embodiment 3

[0044] The method for preparing feed by acidolysis of rice straw, primary neutralization, ammoniation, secondary neutralization, and fermentation of Rhodospira rubrum includes the following steps:

[0045] (1) Raw material handling. Grinding the dry straw with a moisture content of 10.1% into 40 mesh uniform particles;

[0046] (2) Acid hydrolysis. Mix 100 parts of pulverized straw with 150 parts of 1% sulfuric acid solution evenly, heat up to 95°C for 1.5 h, and the process needs to replenish water to the original volume;

[0047] (3) One time neutralization. Add 1.8 parts of CaCO to the above acid hydrolyzate 3 , and stir evenly;

[0048] (4) Amination. Add 250 parts of ammoniacal liquor of 0.3% concentration to above-mentioned neutralization product, and keep airtight at 90 DEG C for 2.5 h;

[0049] (5) Secondary neutralization. The amide is cooled to 36°C, and the pH is adjusted to 7.0 with 1% phosphoric acid solution;

[0050] (6) Fermentation. Add 50 copies of R...

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Abstract

The invention relates to a fodder preparation method converting Rhodospirillum rubrum into various byproducts containing cellulose. The method includes mixing raw materials and acid solution evenly; adding CaCO3 to perform the first neutralization after heating and heat insulation; adding ammonia solution, sealing, insulating heat, cooling, and acquiring PH 7 by adjusting through acid; adding Rhodospirillum rubrum fermentation agent, mixing with cellulase evenly before fermentation for 6 to 7d, and obtaining the Rhodospirillum rubrum fermented solid fodder after drying. The method has the advantages that the cellulose material is degraded moderately, the raw materials are utilized completely to prepare high-protein and low-fiber fodder, the cellulose material value is increased, production cost is reduced, equipment requirement is lowered, and investment is reduced; limited acid hydrolysis is performed on the cellulose material, saccharification and fermentation are integrated into one step, preparation period is shortened, and process is simplified; the generality of the raw material is high, and reference is provided for expanding the wood fiber raw material application range.

Description

technical field [0001] The invention relates to a method for preparing high-protein and low-fiber feed by fermenting and transforming by-products containing cellulose with Rhodospira rubrum. It belongs to the field of recycling and utilizing cellulose-containing waste to produce biological feed products. Background technique [0002] Many industrial and agricultural by-products are rich in cellulose, common ones include liquor waste, straw, sauce residue and vinegar residue, etc. The recycling and reuse of cellulose by-products has become one of the hot and difficult research topics in the world. [0003] Cellulose by-products contain high content of cellulose, hemicellulose and lignin, and there is a complex and strong association between them. Using these raw materials to make products with higher added value (such as feed, etc.), More severe pretreatment methods are often required, which will inevitably increase the process cost and application difficulty. Such as 1.. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18A23K10/38
CPCY02P60/87
Inventor 张健游玲尹礼国朱文优
Owner YIBIN UNIV
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