Rooting culture method of catalpa bungei tissue culture seedling
A technology for rooting culture and tissue culture seedlings, which is applied in the field of plant tissue culture and catalpa tree tissue culture seedlings rooting culture, can solve the problems of difficulty in meeting the requirements of industrial production, low proportion of rooted seedlings, unfavorable industrial production, etc., and achieve saving Operation steps and production costs, reduction of steps, and the effect of promoting industrialization
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Embodiment 1
[0031] Embodiment 1 a kind of catalpa tree tissue culture seedling rooting culture method
[0032] A kind of catalpa tree tissue culture seedling rooting culture method, such as figure 1 As shown, it proceeds in the following order of steps:
[0033] (1) Acquisition of aseptic seedlings of catalpa
[0034] Select strong branches with bud points for hydroponics indoors, cut into 2cm long terminal buds or stem segments with axillary buds after the new buds germinate, cut off the leaves and leave 3mm petioles, wipe the surface of the explants with 75% alcohol, and use Soak in washing powder water for 2 minutes, then rinse under running water for 0.5 hours; on the ultra-clean workbench, treat with 0.05% mercuric chloride for 6 minutes, rinse with sterile water for 5 times, inoculate into medium A, and inoculate 2 strain, to obtain the aseptic seedlings of Catalpa chinensis;
[0035] (2) subculture of sterile vaccines
[0036] Select aseptic seedlings of catalpa with a growth c...
Embodiment 2-6
[0041] Embodiment 2-6 Catalpa tree tissue culture seedling rooting culture method
[0042] Embodiments 2-6 are respectively a method for rooting culture of catalpa seedlings, the steps involved in the culture method are similar to those in Example 1, the only difference is the difference in the technical parameters involved, as shown in the following table Shown:
[0043]
[0044]
[0045] In Example 2-6, medium A uses MS as the base medium, and the added composition is as follows:
[0046]
[0047] In Example 2-6, the medium B uses 1 / 2MS as the base medium, and the added composition is as follows:
[0048]
Embodiment 7
[0049] The screening of embodiment 7 medium A and B
[0050] (1) Screening of Medium A
[0051] Since the same medium is used in the screening of sterile seedlings and subculture, the screening of medium A is particularly important. With MS as the basic medium, 30 g / L of sucrose and 5.8 g / L of agar are added. Different nutrients, but not all growth promoters can promote the growth of catalpa, as shown in the following table:
[0052]
[0053] It can be seen from the above table that when different growth promoters are used for cultivation, the seedling heights, value-added ratios and phenomena of the final cultivated seedlings are all different, indicating that not all growth promoters can be used for cultivation. It has a good effect. Among them, the combination of 6-BA (6-benzyl adenine) and TDZ (thidiazuron) has higher seedling height and value-added ratio than other growth promoters, and the plant differentiation rate is high. The plant height is high, the height is c...
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