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Rooting culture method of catalpa bungei tissue culture seedling

A technology for rooting culture and tissue culture seedlings, which is applied in the field of plant tissue culture and catalpa tree tissue culture seedlings rooting culture, can solve the problems of difficulty in meeting the requirements of industrial production, low proportion of rooted seedlings, unfavorable industrial production, etc., and achieve saving Operation steps and production costs, reduction of steps, and the effect of promoting industrialization

Active Publication Date: 2015-06-24
上海菁艺生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0006] In the above studies on the proliferation medium of catalpa, the hormone ratio is mainly based on the combination of cytokinin and auxin, and the research is mostly at the theoretical level, and the rooted seedlings of tissue culture need to be hardened before transplanting in the greenhouse. In the industrial production process, this step consumes a lot of labor costs, and reducing the cost of hardening seedlings is another difficult problem to be solved in the industrial production of catalpa tissue culture
[0007] Chinese invention patent application CN104054580 A discloses "a kind of tissue culture method that improves catalpa multiplication coefficient", comprises the following steps (1) establishment of sterile culture; (2) induction of axillary buds; (3) proliferation of axillary buds; ( 4) rooting culture of sprouts; (5) hardening and transplanting, five steps, the best axillary bud induction formula for this patent application is MS+BA 0.8mg / L+IBA 0.1mg / L; the best axillary bud proliferation formula is MS+BA1.5mg / L+ZT O.1mg / L+IAA 0.2mg / L; the highest multiplication coefficient is 15, although this method obtains a higher multiplication coefficient, but the multiplication is mainly realized through two steps of axillary bud induction and axillary bud multiplication , if factory production is carried out, the method steps are cumbersome and the labor cost is large, which is not conducive to large-scale factory production
[0008] In the industrial production process of catalpa, it is necessary not only to ensure the expansion ratio, but also to ensure the ratio of seedlings that can be used for rooting. However, most of the existing documents and reports are limited to the theoretical level. The rooting materials need to be selected from the expansion seedlings, which can be used for rooting. The proportion of seedlings is extremely low, which is difficult to meet the requirements of industrial production

Method used

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  • Rooting culture method of catalpa bungei tissue culture seedling
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  • Rooting culture method of catalpa bungei tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 a kind of catalpa tree tissue culture seedling rooting culture method

[0032] A kind of catalpa tree tissue culture seedling rooting culture method, such as figure 1 As shown, it proceeds in the following order of steps:

[0033] (1) Acquisition of aseptic seedlings of catalpa

[0034] Select strong branches with bud points for hydroponics indoors, cut into 2cm long terminal buds or stem segments with axillary buds after the new buds germinate, cut off the leaves and leave 3mm petioles, wipe the surface of the explants with 75% alcohol, and use Soak in washing powder water for 2 minutes, then rinse under running water for 0.5 hours; on the ultra-clean workbench, treat with 0.05% mercuric chloride for 6 minutes, rinse with sterile water for 5 times, inoculate into medium A, and inoculate 2 strain, to obtain the aseptic seedlings of Catalpa chinensis;

[0035] (2) subculture of sterile vaccines

[0036] Select aseptic seedlings of catalpa with a growth c...

Embodiment 2-6

[0041] Embodiment 2-6 Catalpa tree tissue culture seedling rooting culture method

[0042] Embodiments 2-6 are respectively a method for rooting culture of catalpa seedlings, the steps involved in the culture method are similar to those in Example 1, the only difference is the difference in the technical parameters involved, as shown in the following table Shown:

[0043]

[0044]

[0045] In Example 2-6, medium A uses MS as the base medium, and the added composition is as follows:

[0046]

[0047] In Example 2-6, the medium B uses 1 / 2MS as the base medium, and the added composition is as follows:

[0048]

Embodiment 7

[0049] The screening of embodiment 7 medium A and B

[0050] (1) Screening of Medium A

[0051] Since the same medium is used in the screening of sterile seedlings and subculture, the screening of medium A is particularly important. With MS as the basic medium, 30 g / L of sucrose and 5.8 g / L of agar are added. Different nutrients, but not all growth promoters can promote the growth of catalpa, as shown in the following table:

[0052]

[0053] It can be seen from the above table that when different growth promoters are used for cultivation, the seedling heights, value-added ratios and phenomena of the final cultivated seedlings are all different, indicating that not all growth promoters can be used for cultivation. It has a good effect. Among them, the combination of 6-BA (6-benzyl adenine) and TDZ (thidiazuron) has higher seedling height and value-added ratio than other growth promoters, and the plant differentiation rate is high. The plant height is high, the height is c...

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Abstract

The invention discloses a rooting culture method of catalpa bungei tissue culture seedling. The method sequentially comprises the following steps: (1) obtaining aseptic seedlings of the catalpa bungei; (2) subculturing the aseptic seedlings; (3) performing rooting culture of the catalpa bungei tissue culture seedlings; (4) hardening seedlings and transplanting. By adopting the catalpa bungei tissue culture seedling rooting culture method, the propagation ratio is controlled to 1: (3-3.5), the height of the plant of the seedlings is controlled to be 2.5-4cm, and the rooting culture is carried out to guarantee that the rooting proportion is up to 90 percent or above; the production cost is reduced, and the same culture medium is used in the steps of obtaining the aseptic seedlings and subculturing during the rooting culture of the catalpa bungei tissue culture seedling, so that an operation step is eliminated and the production cost is reduced, thus being more suitable for industrial production. According to the rooting culture method of the catalpa bungei tissue culture seedling, the forest tissue culture industrialization for an experiment is realized, the industrialization process is simplified, the steps are reduced, the labor force is controlled, and the cost control is facilitated, thus promoting the industrialization of the woody plant tissue culture. The method is suitable for the rooting culture of the catalpa bungei tissue culture seedling.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for plant tissue culture, in particular to a method for rooting culture of catalpa tree tissue culture seedlings. Background technique [0002] Catalpa bungei C.A.Meyer, belonging to Bignoniaceae and Catalpa genus, is a deciduous tree. Under good site conditions, the height of the tree can reach 30m, and the diameter at breast height can reach more than 1m. However, in terms of reproduction, since the male and female flowering periods of Catalpa do not meet, they are often self-sterile and require artificial pollination to bear fruit. The rate of seed production is very low, the rate of seed germination is not high, and seed reproduction is relatively difficult, resulting in relatively few resources of Catalpa. At present, the root tiller or grafting methods are mostly used to produce catalpa seedlings. With the increase of felling and utilization of catalpa, the conventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冒文娟陈波李文剑刘戈张承妹林大为董举文高畅陈铭苏涌
Owner 上海菁艺生物科技有限公司
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