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Identification method for peanut rod rot resistance of peanut germplasm resources

A technology for peanut fruit rot and resistance identification, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, horticultural methods, etc.

Pending Publication Date: 2015-06-17
SHANDONG PEANUT RES INST
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for identifying the resistance of peanut germplasm resources to fruit rot, so as to overcome technical bottlenecks such as the difficulty of rejoining and quantification of pathogenic mycelia under existing conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 17 peanut varieties were selected as the test varieties, and the field backgrafting test was carried out in the special isolated plot of Qingdao Laixi Peanut Experimental Base. Before planting, the plot should be deeply plowed, evenly turned, harrowed, and leveled, and divided into two plots with inoculation and non-inoculation, and an isolation zone should be set up in the middle. The two plots are divided into 51 plots, each plot is 1.32 m 2 , three replicate treatments were set for each variety, and the cell locations were randomly divided. Double-grain sowing is adopted, the hole spacing is 15cm, the size row spacing is 80cm, 30cm, and the ridge length is 1.65m. Planted on April 28, 2014.

[0016] Insert pathogenic bacteria Calonetria sp. . Before inoculation, first activate the frozen-preserved bacteria on the modified Chapei medium plate, and after activation, insert two 5mm plaques into 50ml modified Chapei liquid medium by punching method, shake at 28°C and ...

Embodiment 2

[0019] The 17 peanut varieties involved in the above field experiments were simultaneously conducted pot experiments at Shandong Peanut Research Institute in Qingdao to verify the stability and reliability of the method. Before planting, the soil was piled up, turned evenly, divided into equal parts, and planted in clay pots with a caliber of 45cm. The double seed sowing method was adopted, and two treatments of inoculation and non-inoculation were set up for each variety, and each treatment was replicated three times, and one replicate was 5 trees. The planting and management modes of each treatment were consistent. Planted on May 5, 2014.

[0020] Same with the above-mentioned peanut fruit rot pathogen Calonetria sp. For the inoculation of bacteria, the breeding method is consistent with the field experiment. The inoculation period was also selected at the seedling stage, full bloom stage and berry stage of peanuts (one week later than the inoculation date of the field te...

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Abstract

The invention relates to an identification method for peanut rod rot resistance of peanut germplasm resources, which belongs to the technical field of identification methods for disease resistance of peanut. According to the method, peanut rod rot pathogen Calonectria sp. is used as an inoculation bacterium and is inoculated in the seedling stage, the full flowering stage and the fruiting stage of peanut through burying and sprinkling irrigation. Homogenization of soil should be carried out before planting of peanut, and peanut is planted in a random planting mode. To guarantee inoculation of equivalent bacterial stain, rapid mass semi-quantitative culture of the pathogen is carried out at first, and the pathogen is inoculated in each inoculation stage according to an equivalent inoculation method. During harvesting, the rotten peanut rates of various varieties are recorded. The identification method provided by the invention can realize identification and comparison of peanut rod rot resistance of a plurality of peanut germplasm resources, has the advantages of accurate and reliable results and good repeatability, and is of great significance to cultivation of peanut rod rot peanut varieties and research on resistance mechanism.

Description

technical field [0001] The invention relates to a method for identifying peanut germplasm resource fruit rot resistance, and belongs to the technical field of peanut disease resistance identification methods. Background technique [0002] Peanut fruit rot disease, commonly known as fruit rot disease, is one of the common soil-borne diseases of peanuts in the world. It is a serious hazard and difficult to control. Generally, the yield loss of the diseased field can reach about 15%, and the severely diseased field can cause no harvest. Screening and breeding of peanut disease-resistant varieties is an effective way to fundamentally realize the prevention and control of fruit rot. [0003] However, due to the difficulty in soil propagation and inoculation of the pathogenic bacteria of peanut fruit rot, especially the quantitative inoculation of non-spore-producing mycelium pathogenic bacteria is extremely difficult, making the identification and comparison of peanut germplasm r...

Claims

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Application Information

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IPC IPC(8): C12Q1/18A01G7/06C12R1/645
Inventor 陈明娜禹山林陈娜潘丽娟迟晓元王冕王通杨珍
Owner SHANDONG PEANUT RES INST
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