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Higher value application method for shaddock agricultural waste resource

An agricultural waste, high-value technology, applied in the direction of fermentation, can solve the problems of low supply of trilobatin, high difficulty, poor operability of the refining process, etc., and achieve low cost, high product quality, and environment-friendly. Effect

Active Publication Date: 2015-06-17
MEIZHOU GOLDEN POMELO HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the plant resources of Polyacaceae are gradually decreasing and the content of trilobatin in other plants is very low. In addition, the refinement process is not easy to operate, so the supply of trilobatin mainly derived from plant extraction is extremely low.
However, the industrial practice of other chemical methods or biotransformation methods is more difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Put 250 kg of crushed and dried Shatian pomelo secondary fruit into a 2-ton extraction tank, add 1500 L of 75% ethanol, heat and reflux for 1 hour; filter the material, and repeat the extraction three times. Inject the extract into a 2T hydrogenation reaction tank, add 150 kg of NaOH and 23 kg of commercial Raney nickel as catalysts, vacuumize and replace with nitrogen, repeat 3 times and then introduce hydrogen, the hydrogen pressure is 1 atmosphere. Then hydrogenate at room temperature for 20 hours. After filtering, the filtrate was adjusted to neutrality with hydrochloric acid in a neutralization tank, and the solid was collected by filtration to obtain 200 kg of wet alkaline hydrogenation product of pomelo flavonoids.

Embodiment 2

[0027] Put 7 kg of the wet alkaline hydrogenation product of pomelo flavonoids obtained above into the enzyme reaction tank, add phosphate buffer solution of pH 6.8 and 5 L rhamnosidase, and react at 30 ° C for 12 h under the condition of slow stirring 100 r / min , and then the solution was heated to 80 degrees, adding activated carbon and stirring for 0.5 hours, filtered and frozen for crystallization. Obtain 4 kg of trilobatin crude product, and obtain a refined product with a content of 99% after recrystallization from ethanol water.

[0028] ESI-MS (m / z): 435[(M-1) + ].

[0029] 1 H NMR (300 MHz, (CD 3 ) 2CO) δ: 2.87 (2H, t), 3.32-3.54 (7H, m), 3.67 (m, 1H), 3.87 (1H, d), 4.35 (2H, s), 4.68 (s, 1H), 4.99 ( 1H, d), 6.11 (s, 2H), 6.71 (2H, d), 7.06 (2H, d), 8.09 (1H, s), 12.36 (2H, s).

Embodiment 3

[0031] Put 7 kg of the wet alkaline hydrogenation product of pomelo flavonoids obtained above into a reaction tank, add 20% ethanol water to dissolve, and react with 20 kg of solid rhamnosidase at 60°C for 4 hours. The resulting reaction solution was released, filtered to remove solid matter, and cooled to crystallize to obtain 4.3 kg of trilobatin with a content of 96%.

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Abstract

The invention discloses a higher value application method for a shaddock agricultural waste resource, in particular to a method for preparing trilobatin form raw materials including waste shaddock fruits and shaddock peels. The method comprises the following steps: extracting shaddock flavone from shaddock industrial waste (including waste shaddock fruits and shaddock peels) in alcohol water, and performing hydrogenation by utilizing a rare metal catalyst under an alkaline condition; and collecting shaddock flavone alkaline hydrogenation products, performing biotransformation by an enzymic method, and obtaining the trilobatin under the certain temperature and conditions. The invention provides a new method for trilobatin industrial production. Furthermore, the utilized raw materials are the waste shaddock fruits and shaddock peels, so that the agriculture ecological resources are activated and protected, and the multi-purpose active material trilobatin obtained by high-efficiency transformation of the waste can be fully utilized. The method is environment-friendly, is suitable for industrial application, and has important significance to the trilobatin industrial production.

Description

technical field [0001] The invention relates to a method for high-value utilization of pomelo agricultural waste resources, in particular to a method for obtaining trilobatin, a component of Duosuke sweet tea, by using discarded pomelo fruit and pomelo peel as raw materials. Background technique [0002] Shatian pomelo is a characteristic crop planted on a large scale in southern my country. During the artificial planting of pomelo, a large number of natural fruit drop (physiological or climatic influences drop pomelo fruit), artificial fruit thinning (artificial fruit thinning to increase the proportion of high-quality fruit) Pomelo fruit), mature and defective fruit, and the peel of fresh fruit after eating, most of them are regarded as garbage and thrown away. These wastes are rich in biologically active components, among which grapefruit flavonoids are one of the available components. Reasonable resource utilization will not only protect the agricultural environment, but ...

Claims

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Application Information

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IPC IPC(8): C12P19/44
Inventor 周金林黄宝华卢宇靖林丽薇
Owner MEIZHOU GOLDEN POMELO HEALTH TECH CO LTD
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