Cordyceps Chinese chestnut

A chestnut and Cordyceps militaris technology, applied in the field of bioengineering, can solve problems affecting the remarkable efficacy of Cordyceps sinensis, and achieve the effects of easy realization of large-scale production, wide application prospects, and simple and feasible methods

Inactive Publication Date: 2015-06-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, cordycepin is mainly present in Cordyceps militaris, and its content in Cordyceps sinensis is very small, but the absence of cordycepin did not affect the significant efficacy of Cordyceps sinensis

Method used

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  • Cordyceps Chinese chestnut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The DS56-Ⅲ twin-screw extrusion extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. was used to process chestnuts. The chestnuts used were commercially available. After peeling, the moisture content was determined to be 45.2% by a moisture analyzer. The chestnut slices were dried, and the moisture content was determined to be 14.5%. The feed rate was set at 120 kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that when the temperatures in the preheating zone, transportation zone, and ripening zone are 60, 160, and 190°C respectively, the expansion degree of chestnuts is the highest, and the chestnuts are dried to different moisture contents. The expansion degree at moisture content. It can be seen from Table 2 that the puffing effect is good when the water content is about 12-20%.

[0019] Table 1: The puffing degree of puffed chestnut obtained by the combination of different temperatu...

Embodiment 2

[0024] Inoculate the Cordyceps sinensis strain into a 250ml Erlenmeyer flask with 50ml medium, the composition of the medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, culture for 96 hours , as seeds for inoculation.

[0025] Inoculate the Cordyceps militaris strain into a 250ml Erlenmeyer flask with 50ml of culture medium. The culture medium is composed of: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, and culture for 96 hours as seeds for inoculation.

[0026] Take puffed chestnuts with a puffing degree of 3.2 and chestnuts with a mechanical crushing degree of 1mm (peeled and dried to a moisture content of 14.5%), mix a total of 50g, add 40g of distilled water, put them into a 500ml Erlenmeyer flask, and sterilize at 121°C After cooling for 20 minutes, inoculate 5ml each of Cordyceps sinensis and Cordyceps militaris seed solution, control the ambient humidity to 90% in a constant temperature and humidity inc...

Embodiment 3

[0030] Take 30g of puffed chestnuts with a puffing degree of 3.2 and 40g of fresh chestnuts (peeled, water content 45.2%) with a mechanical crushing degree of 2mm, mix them, put them into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate into the cultured chestnut after cooling. Each 5ml of Cordyceps sinensis and Cordyceps militaris seed solution was controlled in a constant temperature and humidity incubator with an ambient humidity of 80% and a temperature of 15°C. After 20 days of fermentation, the content of cordycepin was determined to be 4.5%.

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Abstract

The invention discloses a cordyceps Chinese chestnut, and belongs to the field of a bioengineering tehcnology. The cordyceps Chinese chestnut is obtained by taking a Chinese chestnut as a culture medium and carrying out solid state fermentation of mixing double fungus including cordyceps sinensis and cordyceps militaris. According to the cordyceps Chinese chestnut provided by the invention, the Chinese chestnut obtained by the biotransformation of double fungus has the effects of life cultivation and health preservation, thereby having a good application prospect.

Description

technical field [0001] The method adopts Ophiocordyceps sinensis and Cordyceps militaris, uses Chinese chestnut as a culture medium, and performs double-bacteria mixed solid-state fermentation, and belongs to the technical field of bioengineering. The preservation number of the bacterial strain Cordyceps militaris is CICC 14013, and the preservation number of the bacterial strain Ophiocordyceps sinensis is CICC 14088, and the nutrition and health chestnuts are produced through double-bacteria mixed solid-state fermentation. Background technique [0002] Cordyceps belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavcipitaceae . Most of the fungi of the genus Cordyceps are medicinal fungi, among which Ophiocordyceps sinensis is generally known and has the longest application history, and Cordyceps militaris has the most in-depth application research. [0003] According to literature research, Cordyceps sinensis was first recorded in "Yuewang Yaozhe...

Claims

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Application Information

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IPC IPC(8): A23L1/36A23L25/00
Inventor 蔡宇杰杨帆白玉管政兵廖祥儒张大兵
Owner JIANGNAN UNIV
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