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Cordyceps sinensis cassava

A technology of cassava and Cordyceps militaris, applied in the field of bioengineering, can solve the problems affecting the remarkable efficacy of Cordyceps sinensis, and achieve the effect of easy to realize large-scale production, controllable quality, simple and feasible method

Inactive Publication Date: 2015-08-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, cordycepin is mainly present in Cordyceps militaris, and its content in Cordyceps sinensis is very small, but the absence of cordycepin did not affect the significant efficacy of Cordyceps sinensis

Method used

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  • Cordyceps sinensis cassava

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The DS56-Ⅲ twin-screw extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. was used to process cassava. The cassava used was commercially available, and its moisture content was 71.2% as measured by a moisture analyzer. The cassava slices were dried and the moisture content was determined to be 13.8%. Set the feed rate to 120kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that the expansion degree of cassava is the highest when the temperature of the preheating zone, the transportation zone and the maturation zone are 60, 160, and 190℃ respectively. The cassava is dried to different moisture content, and the different conditions are investigated under this temperature. The degree of puffing at the moisture content. It can be seen from Table 2 that the expansion effect is good when the moisture content is about 12-20%.

[0019] Table 1: The degree of expansion of the expanded cassava obtai...

Embodiment 2

[0024] Inoculate the Cordyceps strain into a 250ml Erlenmeyer flask containing 50ml culture medium. The composition of the culture medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25℃, shaker speed 150 revolutions / min, culture for 96 hours , As a seed for inoculation.

[0025] The Cordyceps militaris strain was inoculated into a 250ml Erlenmeyer flask containing 50ml medium. The medium composition was: glucose 20g / l, potato juice 200g / l, pH natural, 25℃, shaker speed 150 rpm, culture 96 Hours, as seeds for inoculation.

[0026] Take the puffed cassava with a puffing degree of 5.7 and the cassava with a mechanical pulverization degree of 1mm (which has been dried to a moisture content of 13.8%) and mix, total 50g, then add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, After cooling, inoculate 5ml each of Cordyceps sinensis and Cordyceps militaris seed liquid, control the environmental humidity in a constant temperature and hum...

Embodiment 3

[0030] Take 30g of puffed cassava with a puffing degree of 5.7 and 70g of fresh cassava with a mechanical pulverization degree of 2mm (water content 71.2%), put them into a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes, and inoculate the cultivated cordyceps and pupae after cooling. Cordyceps seed liquid was 5ml each, the environmental humidity was controlled at 80% in a constant temperature and humidity incubator, the temperature was 15°C, and the cordycepin content was determined to be 4.2% after 20 days of fermentation.

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Abstract

With cassavas as a culture medium, cordyceps sinensis and cordyceps militaris are subjected to double-bacterial mixed solid fermentation. The invention belongs to the technical field of biological engineering. The cassavas prepared through the double-bacterial bio-conversion have functions of health caring and are excellent in application prospect.

Description

Technical field [0001] Using Cordyceps sinensis (Ophiocordyceps sinensis) and Cordyceps militaris (Cordyceps militaris), using cassava as a medium, a two-strain mixed solid-state fermentation is performed, which belongs to the technical field of bioengineering. The preservation number of strain Cordyceps militaris is CICC 14013, and the preservation number of strain Ophiocordyceps sinensis is CICC 14088, and nutrient health cassava is produced by two-strain mixed solid-state fermentation. Background technique [0002] Cordyceps belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavicipaceae . Most of Cordyceps fungi are medicinal fungi. Among them, Cordyceps sinensis (Ophiocordyceps sinensis) is generally well known and has the longest application history, and Cordyceps militaris has the most in-depth application research. [0003] Cordyceps sinensis was first recorded in "Yuewang Medicine Diagnosis" in 710 AD. It was first seen in Wu Yiluo's "Materia Med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/214A23L1/29A01G1/04A23L19/10A23L33/00
Inventor 蔡宇杰杨腾义白玉管政兵廖祥儒张大兵
Owner JIANGNAN UNIV
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