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Colorimetric method for detecting lead ions

A technology of lead ion and colorimetry, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of difficult on-site in-situ detection and high sensitivity, and achieve the effect of easy operation, high sensitivity and good selectivity

Inactive Publication Date: 2015-05-27
王利兵
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  • Claims
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Problems solved by technology

In recent years, colorimetric methods have been used to detect Pb 2+ There are many studies [Huang K W, Yu C J, Tseng W L. Biosensors and Bioelectronics, 2010, 25 (5): 984-989; Chai F, Wang C, Wang T, et al. ACS applied materials & interfaces, 2010, 2 ( 5): 1466-1470], but most of these methods cannot meet the three requirements of simple and cheap, high sensitivity and good selectivity at the same time, and it is difficult to apply in-situ detection

Method used

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preparation example Construction

[0031] 2. Preparation of Cys-modified AuNPs solution

[0032] After centrifuging 1 mL of AuNPs solution at 12000 rpm for 20 min, the supernatant was sucked off and resuspended in 900 μL of ultrapure water, and 10 μL of 5 μM freshly prepared Cys solution was added, shaken and left to incubate for 0.5 h. figure 1 (B) It can be shown that Cys has been modified to the surface of AuNPs, and Cys–AuNPs still exist in the solution in a monodisperse form.

[0033] 3. New Pb 2+ Configuration of standard solution

[0034] Take 1 mL of Pb with different concentrations of 0, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100 μM 2+ Add 20μL of 10mM potassium thiocyanate solution to the standard solution to make new Pb 2+ standard solution.

[0035] 4. Pb 2+ determination

[0036] 1. Detection sensitivity

[0037] Add 0, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 μM standard Pb to the above 900 μL Cys–AuNPs solution 2+ After the solution was incubated for 30 minutes, the spectrum was obtained by scannin...

Embodiment 1

[0041] 13nm AuNPs were prepared by reducing HAuCl with sodium citrate according to the literature method 4 synthetic method. Add 10 μL of 5 μM freshly prepared Cys solution to 900 μL of the reconstituted AuNPs solution, shake and incubate for 0.5 h. Take 1mL of drinking water and add 10μL of 100μM Pb 2+ Standard solution and 2μL 10mM potassium thiocyanate solution. Add 100 μL of the above solution to 0.9 mL of Cys–AuNPs solution, and after incubation for 30 minutes, scan and record the spectrum in the wavelength range of 400-800 nm. It was found that the standard deviation of 3 measurements was 0.56%, and the average recovery was 95.7%, which indicated that the system could be used for Pb in drinking water 2+ detection.

Embodiment 2

[0043] Take 1mL drinking water, add 10μL 1mM standard Pb 2+ solution and 2 μL of 10 mM potassium thiocyanate solution. Add 100 μL of the above solution to 0.9 mL of Cys–AuNPs solution, and after incubation for 30 minutes, scan and record the spectrum in the wavelength range of 400-800 nm. The standard deviation of 3 measurements is 0.346%, and the average recovery rate is 102.4%, which indicates that the system can be used for Pb in drinking water 2+ detection.

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Abstract

The invention discloses a colorimetric method for detecting lead ions, and specifically relates to a method for detecting lead ions by taking gold nanoparticles as a colorimetric probe. AuNPs(Cys-AuNPs) is modified by l-cysteine, and Cys can capture Pb<2+> by the binding function of a chelating ligand under the induction of Pb<2+>, so that Cys-AuNPs is aggregated, and thus the color of a solution changes, and the change in position and intensity of an AuNPs characteristic peak is caused. The colorimetric method has a detection range of 0.02- 5 microns, and detection limit of 0.02 microns, has the advantages of simplicity, economy, good selectivity (potassium thiocyanate as a screening agent) and the like, and can be used for detecting Pb<2+>.

Description

technical field [0001] The invention belongs to a method for detecting heavy metal ions, in particular to a method for detecting lead ions by using a colorimetric method, in particular to a method for detecting lead ions using gold nanoparticles as a colorimetric probe. Background technique [0002] Toxic metal ions will have a serious impact on the environment and human health, especially lead ions, which can also have a strong toxic effect on human nerves, blood, digestion and kidneys in a small amount of contact, affecting human behavior, brain development and Nerve conduction velocity. In 2006, the Ministry of Health promulgated the new version of "Drinking Water Hygienic Standards" (GB5749-2006), which stipulates that the lead content in daily drinking water shall not be higher than 0.01mg / L (equivalent to 0.05μM). Therefore, establishing an accurate and rapid lead detection method has important application value and practical significance. [0003] The detection meth...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 王利兵丁利成婧许宙梅博朱绍华龚强
Owner 王利兵
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