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Method for preparing antihypertensive peptides by fermenting marine organisms through probiotics

A technology for marine organisms and blood pressure-lowering peptides, applied in the biological field, can solve problems such as no blood pressure-lowering peptides, and achieve good application prospects, social and economic benefits, and efficiency improvements.

Inactive Publication Date: 2015-05-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the preparation of blood pressure-lowering peptides using collagen-rich marine organisms as raw materials, using a technical solution that combines protease liquefaction and probiotic fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: A method for preparing hypotensive peptides by fermenting sea cucumbers with probiotics, the specific process steps are as follows:

[0021] a. Selection and pretreatment of raw materials: select 100kg fresh sea cucumbers, remove the intestines, put them in boiling water and cook for 20 minutes, remove the water and remove the mouth, and then soften them in a sterilizing pot at 0.1MPa, 121°C, and 30 minutes to soften the sea cucumbers It was 7kg, then soaked in clear water to remove the salt, washed and drained, used a beater for beating, added 200L of pure water, and used a colloid mill to grind and refine to obtain a slurry;

[0022] b. Protease liquefaction: heat the obtained slurry until the center temperature reaches 100°C, keep it for 10 minutes, kill microorganisms, cool to 50°C, adjust the pH to 5.2 with HCl, add 40g of papain for liquefaction treatment for 2 hours, then heat the liquefied liquid When the central temperature reaches 100°C, keep it f...

Embodiment 2

[0027] Example 2: A method for preparing hypotensive peptides by fermenting sea cucumbers with probiotics, the specific process steps are as follows:

[0028] a. Selection and pretreatment of raw materials: Select 5kg of dried sea cucumbers, soak them in cold water for 1 day, remove them and boil them in boiling water for 20 minutes, soften them in a sterilizing pot at 0.1MPa, 121°C for 30 minutes, and then soak them in clean water Remove the salt, wash, remove and drain the water, use a beater for beating treatment, add 100L of pure water, and use a colloid mill for grinding and refining treatment to obtain a slurry;

[0029] b. Protease liquefaction: heat the obtained slurry until the central temperature reaches 95°C, keep it for 10 minutes, kill microorganisms, cool to 55°C, add 50g flavored protease for liquefaction treatment for 1 hour, heat the liquefied liquid until the central temperature reaches 95°C, Keep it for 10 minutes to inactivate the enzyme activity to obtai...

Embodiment 3

[0034] Example 3: A method for preparing hypotensive peptides by fermenting jellyfish with probiotics, the specific process steps are as follows:

[0035] a. Selection and pretreatment of raw materials: Select 100kg of fresh jellyfish, soak it in water to remove the salt, wash it and remove it to drain the water, use a beater for beating treatment, add 200L of pure water, and use a colloid mill for grinding and refining treatment to obtain slurry;

[0036] b. Protease liquefaction: heat the obtained slurry until the central temperature reaches 100°C, keep it for 10 minutes, kill microorganisms, cool to 50°C, add 50g flavored protease for liquefaction treatment for 2 hours, then heat the liquefied liquid until the central temperature reaches 100°C, Keep it for 10 minutes to inactivate the enzyme activity to obtain an enzyme inactivated liquefaction solution;

[0037] c. Preparation of the initial fermentation broth: add 2 kg of glucose to the obtained enzyme-inactivated liq...

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Abstract

The invention relates to a method for preparing antihypertensive peptides by fermenting marine organisms through probiotics. The method comprises the following steps: with marine organisms, such as sea cucumber, jellyfish, acaudina molpadioidea or sleeve-fish, rich in collagen, as raw materials, desalting, cleaning, carrying out pulping and grinding treatment, and heating for killing microorganisms; cooling, adding protease, carrying out liquefaction treatment, carrying out enzyme deactivation, adding glucose, and then putting into a fermentation tank; sterilizing, inoculating a probiotic leavening agent, fermenting, and filtering out residues; adding a cryoprotectant to fermented filtrate, carrying out freeze-drying to directly obtain a composite antihypertensive biological preparation containing active probiotics and bioactive peptides; and in addition, further carrying out tubular membrane purification, ultrafiltration membrane intercept classification and nanofiltration membrane concentration and desalting on the fermented filtrate, and carrying out spray drying or freeze-drying to obtain antihypertensive peptide products with relatively high purity. The marine organisms rich in collagen are adopted as the raw materials; the technical scheme of combining protease liquefaction with probiotic fermentation is adopted. Compared with a traditional enzymatic preparation technology, the method has the advantages that the preparation efficiency of high-activity antihypertensive oligopeptide is improved and enhanced; and the preparation technology is reasonable and effective, and is high in operability and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, it is a method for preparing hypotensive peptides by using probiotics to ferment marine organisms. Background technique [0002] Hypertension is a common chronic cardiovascular disease with a long incubation period and many complications. It requires long-term drug treatment to control the development of the disease. At present, although synthetic antihypertensive drugs have remarkable curative effects, they have produced various side effects in the process of clinical application. Therefore, the hypotensive functional factors existing in natural animal and plant resources have received extensive attention at home and abroad. Among them, antihypertensive peptides have become a hot spot in the research of prevention and treatment of hypertension today, and they are safe, effective, and non-toxic and side effects. [0003] At present, the preparation methods of hypotensive peptide main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/39A61P9/12
Inventor 周鹏陈卫刘小鸣刘大松胡锦华
Owner JIANGNAN UNIV
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