Method for preparing novel silkie bone polypeptides
A new technology of black-bone chicken bone, which is applied in the field of preparation of new black-bone chicken bone polypeptide, can solve the problems of being difficult to decompose and damage the environment, and achieve the effects of retaining biologically active substances, easy absorption, and mild reaction conditions
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Embodiment 1
[0021] A preparation method of a novel black-bone chicken bone polypeptide, comprising the steps of:
[0022] (1) Degreasing the black-bone chicken bones: steam the black-bone chicken bones in boiling water for 15 minutes, remove and drain for later use;
[0023] (2) black-bone chicken bone crushing: the black-bone chicken bone processed in step (1) is pulverized by a pulverizer, and sieved with 60 orders;
[0024] (3) Enzymolysis of black-bone chicken bone: step (2) put the crushed black-bone chicken bone powder and water into the container at a ratio of 100g / L, keep the temperature of the enzymatic hydrolysis solution at 40°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic hydrolysis Add trypsin to the volume of the solution to ensure that the concentration of trypsin in the enzymolysis solution is 2.5g / L, adjust the pH of the enzymolysis solution to 8, and adjust the pH of the enzymolysis solution to 8 every 15 minutes. After ...
Embodiment 2
[0030] Compared with embodiment one, its difference is:
[0031] Step (3) Enzymolysis of black-bone chicken bone: In step (2), put the crushed black-bone chicken bone powder and water into the container at a ratio of 150g / L, keep the temperature of the enzymatic hydrolysis solution at 50°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic Add trypsin to the volume of the hydrolysis solution to ensure that the concentration of trypsin in the enzymolysis solution is 2.5g / L, adjust the pH of the enzymolysis solution to 8.5, and adjust the pH of the enzymolysis solution to 8.5 every 15 minutes. After 4.5 hours of enzymolysis, The enzymolysis liquid is heated to boiling, and the resulting hydrolysis degree is 15.63%.
Embodiment 3
[0033] Compared with embodiment one, its difference is:
[0034] Step (3) Enzymolysis of black-bone chicken bone: In step (2), put the crushed black-bone chicken bone powder and water into a container at a ratio of 200g / L, keep the temperature of the enzymatic hydrolysis solution at 55°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic Add trypsin to the volume of the hydrolysis solution to ensure that the concentration of trypsin in the enzymolysis solution is 4g / L, adjust the pH of the enzymolysis solution to 9, and adjust the pH of the enzymolysis solution to 9 every 15 minutes. After 6 hours of enzymolysis, the enzyme The solution was heated to boiling, and the obtained degree of hydrolysis was 14.78%.
[0035] According to the process of Examples 1 to 3, the black-bone chicken bone polypeptide powder is mixed with red date paste, pearl powder, sucrose, and water in a weight ratio of 2:3:1:1:10, and a polypeptide that promotes...
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