Tobacco cellulose content determination method

Abstract

The invention discloses a tobacco cellulose content determination method, which is characterized by comprising the following steps: firstly, weighing different mass of cellulose standard sample powder, adding the powder into sample tubes which use silicone tubes as internal standard substance, testing in a carbon 13 cross polarization magic angle spinning nuclear magnetic resonance spectrometer, and thus obtaining wave spectrogram of each cellulose standard sample, processing with FeakFit software to obtain a ratio of quantitative peak area of the standard sample in the same wave spectrogram to the quantitative peak area of the internal standard substance, by taking absolute mass of the cellulose in the standard sample as abscissa and taking the ratio as ordinate for drawing, obtaining a standard work curve; then, repeating the steps above, obtaining the ratio of the quantitative peak area of a to-be-determined sample in a to-be-determined tobacco sample wave spectrogram to the quantitative peak area of the internal standard substance, further obtaining absolute mass of the cellulose in the to-be-determined tobacco sample according to the standard work curve, thereby obtaining the cellulose content in the to-be-determined tobacco sample. The determination method needs no pretreatment, is accurate in quantification, and is applicable to determination of tobacco cellulose content in batches.

Description

technical field [0001] The invention relates to a method for measuring tobacco cellulose content, which belongs to the field of tobacco analytical chemistry research. Background technique [0002] Tobacco cellulose is an important tobacco biopolymer that mainly plays a role of mechanical support during the growth of tobacco plants. Cellulose has a direct impact on the combustion performance of tobacco leaves and the taste of cigarettes. Tobacco leaves with high cellulose content have good combustibility and enhanced fire holding power, but after burning, they endow the smoke with sharp irritation and the smell of "burning paper". Therefore, tobacco cellulose content is a valuable parameter to characterize cigarette products, and accurate quantification of cellulose content in tobacco samples is of great significance for cigarette formulation design. [0003] At present, the tobacco industry basically uses chemical methods to determine the cellulose content in tobacco, that ...

AI Technical Summary

Problems solved by technology

However, because tobacco is a very complex chemical system, in order to eliminate the interference of other substances on cellulose, Hall and Wooten first put the tobacco samples to be tested in hot water for washing to obtain water-insoluble and water-soluble substances. , and then these two types of components are dried, and the NMR spectra of the water-insoluble and water-soluble substances are tested respectively. During data processing, the NMR spectra of the water-soluble substances are used as the background from the However, in the research process of Hall and Wooten, no suitable internal standard for solid-state sample detection was found, so as to eliminate the instrument The influence of the fluctuation of its own signal on the detection results has led to the unsatisfactory correlation coefficient of its standard working curve; in addition, in the process of testing different samples, in order to save pre-processing time, Hall and Wooten both compared the water-soluble substances of the same sample The NMR spectrum is used as the test background for all samples, which will also bring systematic errors to the measurement results

Therefore, its specific application has been greatly limited

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