Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzyme-free potentiometric glucose sensor and detection method thereof

A glucose sensor and potentiometric technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of cumbersome operation, high cost, unsuitable rapid detection of glucose, etc., and achieves the effects of low cost, simple production, and easy miniaturization.

Inactive Publication Date: 2015-05-06
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many boric acid-based glucose sensors have been developed. In addition to using boric acid derivatives as glucose recognition molecules, such sensors usually require a reporter molecule to conduct the recognition process; although some sensors do not require such reporter molecules, their Most of the spectral signal readout methods used require the help of complex optical equipment, so the operation is cumbersome and the cost is high, and it is not suitable for simple and rapid detection of glucose.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme-free potentiometric glucose sensor and detection method thereof
  • Enzyme-free potentiometric glucose sensor and detection method thereof
  • Enzyme-free potentiometric glucose sensor and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present invention uses [methylene-bis(3,1-phenylaminomethyl-2,1-phenyl)] diboronic acid (see figure 2 ) is used as an example to detect glucose as a recognition molecule, which can be prepared according to the description in Document 5. The operation steps are as follows:

[0029] (1) Preparation of polymer membrane electrodes

[0030] Weigh 196mg of polyvinyl chloride, 196mg of o-nitrophenyloctyl ether, and 4.54mg of tridodecylmethylammonium chloride, dissolve them in 3.5mL of tetrahydrofuran, stir them evenly, and pour them into a glass plate with an inner diameter of 3.5cm. in the glass ring. After the tetrahydrofuran was volatilized, the membrane was cut into small discs with an inner diameter of 3 mm using a puncher and pasted on the tip of a pipette with a PVC tube at the bottom. Use 10mM NaCl solution as the inner filling solution and activation solution to activate the electrode, ready for use.

[0031] (2) Optimization of pH conditions

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Belonging to the technical field of biochemical analytical test, the invention specifically relates to an enzyme-free potentiometric glucose sensor with high selectivity and a detection method thereof. A boric acid derivative containing two boric acid groups is used as a glucose recognition molecule, and a quaternary ammonium salt doped polymer is taken as the electrode. The sensor provided by the invention is employed to detect the concentration of glucose in a whole blood sample, and the obtained result is consistent with that of commercially available glucometers.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis and testing, and in particular relates to a high-selectivity, enzyme-free potentiometric glucose sensor and a detection method thereof. Background technique [0002] Sugar has important physiological significance in the life activities of the human body, and abnormal blood sugar concentration is often used as a sign of metabolic disorders, so the detection of blood sugar is of great significance. In clinical analysis and actual operation, blood glucose detection is generally completed with the aid of a blood glucose meter. The blood glucose meter uses glucose oxidase to recognize and catalyze the oxidation of glucose to generate electroactive hydrogen peroxide, and uses a miniaturized electrode to detect the redox current of hydrogen peroxide, which indirectly realizes the detection of glucose. Although such sensors usually have good selectivity and sensitivity, due to the variabilit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/416
Inventor 秦伟李龙陈红朵
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com