Illumination regulating and controlling composition for specifically regulating and controlling internuncial neuron activity of hypothalamus and application of illumination regulating and controlling composition

A technology of interneurons and hypothalamus, applied in the field of light-regulating compositions

Active Publication Date: 2015-05-06
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] However, there is currently no research report on the specific regulation of interneurons using light-sensing gene regulation technology

Method used

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  • Illumination regulating and controlling composition for specifically regulating and controlling internuncial neuron activity of hypothalamus and application of illumination regulating and controlling composition
  • Illumination regulating and controlling composition for specifically regulating and controlling internuncial neuron activity of hypothalamus and application of illumination regulating and controlling composition
  • Illumination regulating and controlling composition for specifically regulating and controlling internuncial neuron activity of hypothalamus and application of illumination regulating and controlling composition

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Embodiment 1

[0032]The scheme of this embodiment mainly includes the following aspects: 1) Construct a virus vector carrying a light-sensitive gene and infect the interneurons of the hypothalamus; 2) Stimulate the interneurons or projection terminals of the hypothalamus with 473nm blue light and record Photocurrent, so as to verify the excitatory effect of optogene on interneurons; 3) Study the regulation of anxiety-like behavior after regulating the activity of interneurons by in vivo photostimulation, and further use the mouse model of anxiety, through The in vivo activity of specific frequency-affected neurons was used to assess the effect on the severity of anxiety behaviors. The specific implementation is as follows:

[0033] 1) Construct a viral vector carrying an optogene and infect interneurons

[0034] The vector carrying the photosensitive gene (ChR2 plasmid, product of Invitrogen Company), green fluorescent gene (GFP, product of Invitrogen Company) and interneuron-specific prom...

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Abstract

The invention provides an illumination regulating and controlling composition for specifically regulating and controlling internuncial neuron activity of hypothalamus and an application of the illumination regulating and controlling composition. The internuncial neuron activity of the hypothalamus is specifically regulated and controlled by an optogenetic regulation and control technology to affect an anxiety behavior. The illumination regulating and controlling composition for specifically regulating and controlling the internuncial neuron activity of the hypothalamus comprises a virus vector and an illumination device, wherein the virus vector is used for infecting photoesthetic genes carried by internuncial neurons or precursor cells of the internuncial neurons; the carrier comprises a promoter, the photoesthetic genes and a green fluorescence marker gene; the promoter is a VGAT promoter; and the illumination device is used for carrying out illumination regulation and control on the infected internuncial neuron cells or precursor cells of the internuncial neuron cells to change the activity. According to the technique provided by the invention, the internuncial neurons of the hypothalamus can be accurately and specifically regulated and controlled; and anxiety-like behaviors can be effectively affected.

Description

technical field [0001] The invention relates to a light regulation composition for specifically regulating the activity of hypothalamic interneurons and an application thereof. The activity of hypothalamic interneurons is specifically regulated through light-sensing gene regulation technology to affect anxiety behavior. Background technique [0002] In some large and medium-sized cities in my country, with the development of the economy, industry competition is becoming more and more fierce, resulting in an increase in the total prevalence of mental illness among residents. Taking Shenzhen as an example, the total prevalence of mental illness among residents has reached 21.19%, the highest in the country. It ranks first in the total burden of diseases, surpassing malignant tumors, cardiovascular and cerebrovascular diseases and other diseases. Among mental illnesses, the incidence of depression is gradually increasing. At present, there are no less than 100,000 patients with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/86C12N13/00C12N5/10A01K67/027
Inventor 杨帆刘运辉屠洁王立平
Owner SHENZHEN INST OF ADVANCED TECH
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