Method for detecting quality of moghania macrophylla

A quality inspection method and technology of large-leaf extraction, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection limit, complex preparation method of test sample, low recovery rate, etc., and achieve easy operation and time saving. and labor cost, the effect of short time

Active Publication Date: 2015-04-29
ZHUZHOU QIANJIN PHARMA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0015] In the above method, the preparation method of the test product is complicated, the recovery rate is low, and the minimum detection limit is high

Method used

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  • Method for detecting quality of moghania macrophylla
  • Method for detecting quality of moghania macrophylla
  • Method for detecting quality of moghania macrophylla

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: detection

[0068] 1 Materials, equipment and reagents

[0069] 1.1 Materials:

[0070] Daye Qianjinba sample: from Guangxi, sampling time is April 20, 2014.

[0071] Reference substance: Genistein (for content determination, batch number: MUST-120220707) was purchased from Beijing Hengyuan Qitian Chemical Technology Research Institute; genistein (for content determination, batch number 111704-200501) was purchased from China Pharmaceutical Biological Testing institute.

[0072] Control medicinal materials: purchased from China National Institutes for Food and Drug Control, specification: 2.5g.

[0073] 1.2 Equipment

[0074] Agilent Thchnologies 1200series high performance liquid chromatography (Agilent Technologies Co., Ltd.);

[0075] AB204-S 1 / 100,000 electronic analytical balance (German Mettler-Toledo Instrument Co., Ltd.);

[0076] KQ5200DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.);

[0077] FZ102 miniature plant pulver...

Embodiment 2

[0190] 1. Comparative example 1: Take 2 g of the Daye Qianjinba medical material powder (passed through a No. 4 sieve) in the present invention, prepare the test solution according to the preparation method of the test solution in the content of CN201410015559.1, and press CN201410015559. 1 The content of genistin and genistein is determined by the chromatographic conditions in the summary of the invention.

[0191] 2. Get 2 g of Daye Qianjinba medical material powder (crossing No. 4 sieve) in the present invention according to the preparation method of need testing solution in the embodiment of the present invention (method one, method two, method three, method four, method five, method Six, method seven, method eight, method nine) Prepare the test solution, and measure the content of genistin and genistein according to the chromatographic conditions in the content of the invention of CN201410015559.1.

[0192] The experimental results are shown in Table 9:

[0193] Table 9 ...

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Abstract

The invention relates to a method for detecting the quality of moghania macrophylla. The method comprises identification and content measurement. Compared with the prior art, the method for detecting the quality of the common traditional Chinese medicament, moghania macrophylla, is high in stability and sensitivity, and can authenticate the original medicinal materials more simply and intuitively, so as to provide a basis for the qualitative and quantitative detection of the moghania macrophylla. Moreover, the quality and the curative effect of the moghania macrophylla can be guaranteed better, and the medicinal resources of the moghania macrophylla can be utilized better.

Description

technical field [0001] The invention relates to a quality detection method of traditional Chinese medicine, in particular to a quality detection method of Daye Qianjinba. Background technique [0002] Flemingia macrophylla (Willd.) Prain. is the dried root of Flemingia macrophylla (Willd.) Prain. in Papilionaceae. The quality standard of the original plant of Qianjinba, Daye Qianjinba, is only the description of the properties of the medicinal material and the records of its microscopic characteristics, which has a great influence on the development of new drugs. [0003] Plants of the genus Echinacea mainly contain flavonoids, terpenes, steroids and other components, among which flavonoids are the main substances, most of which are isoflavones, and isoflavones substituted by isopentenyl account for the majority. [0004] In recent years, with the deepening of research, more and more medicinal materials are used in various clinical preparations. It has strong pharmacologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/90
Inventor 龚云张鹏林鹏飞彭开锋
Owner ZHUZHOU QIANJIN PHARMA
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