Nanoscale resolution measurement method for three-dimensional position of particles in liquid environment

Abstract

The invention relates to the field of digital holographic microscopic measurement and the field of particle position measurement, and aims to propose a method based on coaxial digital holographic microscopic technology and image processing to realize nanoscale resolution measurement of three-dimensional position of multi-particles in a complex liquid environment. For this reason, the technical solution adopted by the present invention is that the method for measuring the nanoscale resolution of the three-dimensional position of the particle in a liquid environment includes the following steps: using a coaxial digital holographic microscopic imaging system to track the particle in real time and record the holographic image of the particle; Carry out preprocessing on the hologram; carry out holographic reconstruction on the hologram obtained by the above processing; perform deconvolution operation on the reconstruction information obtained above; use the centroid method to obtain the lateral position of the particle; through the position of the particle center of mass obtained above, get Its intensity distribution curve in the longitudinal direction; the above curve is fitted by polynomial fitting, and the peak value is the position of the particle in the longitudinal direction. The invention is mainly applied to microscopic measurement.

Description

technical field [0001] The invention relates to the field of digital holographic microscopic measurement and the field of particle position measurement. Specifically, it is a method for measuring the three-dimensional position of particles in a liquid environment at the nanoscale using digital holographic microscopic measurement technology, and particularly relates to the three-dimensional particle position in a liquid environment. Position nanoscale resolution measurement method. Background technique [0002] Particles generally refer to particles ranging in size from submicron to hundreds of microns, including biological particles such as cells and viruses, as well as non-biological particles such as polystyrene beads, magnetic beads, silicon spheres, colloids, and fluorescent particles. Micron-long rods, etc. The measurement technology of the three-dimensional position of particles in the liquid environment has always been an important subject of the measurement discipli...

AI Technical Summary

Problems solved by technology

The resolution of the Mie scattering measurement method can reach 1nm, but it needs to accurately calibrate various physical parameters, the measurement method is complex, and can only measure regular particles; while the measurement resolution of other methods is about 10nm, which cannot meet the measurement requirements

The above method cannot measure multiple particles at the same time, the measurement efficiency is low, and it cannot measure overlapping particles in the liquid environment, and its robustness is low

Images