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Production method of soy isoflavone with high content of genistin

A technology of soybean isoflavones and genistin, which is applied in the field of production of soybean isoflavones with high genistin content, can solve the problem of the decline of medical and health care reputation of soybean isoflavones, the purity of soybean isoflavones is not exceeded, and the estrogen-like effect is not obvious, etc. problems, to achieve the effect of improving the development of female secondary sexual characteristics, preventing and treating menopausal syndrome, and preventing and treating cancers related to estrogen secretion

Active Publication Date: 2015-04-29
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purity of soy isoflavones produced by the above method does not exceed 80%, the content of genistin (G) is low, and the estrogen-like effect is not obvious, which leads to the decline of the reputation of soy isoflavones in medical care and threatens the decline of the soy isoflavones industry

Method used

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  • Production method of soy isoflavone with high content of genistin
  • Production method of soy isoflavone with high content of genistin
  • Production method of soy isoflavone with high content of genistin

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Raw material soybean meal crushing

[0033] Using soybean meal with protein content ≥ 50%, residual fat ≤ 2.5%, and NSI value ≥ 80% as raw material, crush soybean meal into 20 mesh powder;

[0034] 2. Ethanol leaching

[0035] Put the powder obtained in step 1 into an extraction tank with a stirrer, add ethanol with a concentration of 55% into the extraction tank, add a solvent amount that is 10 times the weight of soybean meal powder, stir fully, and the stirring speed is 30r / min , stirring time 4h;

[0036] 3. Screening

[0037] Filter the mixed material liquid obtained in step 2 through a 60-mesh sieving machine, the power of the conveying material pump is 10KW, and the working pressure is 0.2Mpa, and the liquid phase and solid phase are screened; the solid phase is sent to the automatic friction desolventizer Dry, recover ethanol, and recycle for production; the obtained dry matter with a moisture content of ≤10% is used as the first by-product "highly denatu...

Embodiment 2

[0049] 1. Raw material soybean meal crushing

[0050] Using soybean meal with protein content ≥ 50%, residual fat ≤ 2.5%, and NSI value ≥ 80% as raw material, crush soybean meal into 50 mesh powder;

[0051] 2. Ethanol leaching

[0052] Put the powder obtained in step 1 into an extraction tank with a stirrer, add ethanol with a concentration of 65% into the extraction tank, add a solvent amount that is 5 times the weight of soybean meal powder, stir fully, and the stirring speed is 60r / min , stirring time 6h;

[0053] 3. Screening

[0054] Filter the mixed material liquid obtained in step 2 through a 60-mesh sieving machine, the power of the conveying material pump is 10KW, and the working pressure is 0.4Mpa, and the liquid phase and solid phase are sieved; the solid phase is sent to the automatic friction desolventizer Dry, recover ethanol, and recycle for production; the obtained dry matter with a moisture content of ≤10% is used as the first by-product of this patent, "...

Embodiment 3

[0066] 1. Raw material soybean meal crushing

[0067] Using soybean meal with protein content ≥ 50%, residual fat ≤ 2.5%, and NSI value ≥ 80% as raw material, crush soybean meal into 40 mesh powder;

[0068] 2. Ethanol leaching

[0069] Put the powder obtained in step 1 into an extraction tank with a stirrer, add ethanol with a concentration of 60% into the extraction tank, add a solvent amount that is 8 times the weight of soybean meal powder, stir fully, and the stirring speed is 50r / min , stirring time 5h;

[0070] 3. Screening

[0071] Filter the mixed material liquid obtained in step 2 through a 60-mesh sieving machine, the power of the conveying material pump is 10KW, and the working pressure is 0.3Mpa, and the liquid phase and solid phase are sieved; the solid phase is sent to the automatic friction desolventizer Dry, recover ethanol, and recycle for production; the obtained dry matter with a moisture content of ≤10% is used as the first by-product of this patent, "...

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Abstract

The invention discloses a production method of soy isoflavone with high content of genistin. The production method comprises the following steps: crushing a raw material namely soybean meal, leaching by using ethanol, filtering and screening by using a screening machine, distilling to recover ethanol, precipitating isoflavone by using n-butyl alcohol, filtering to separate out a precipitation solid-phase material generated by the isoflavone in an n-butyl alcohol solution, performing washing separation and purification for the first time, and performing washing separation and purification for the second time. The production method disclosed by the invention has the positive effects that the purity of the obtained soy isoflavone is more than or equal to 90% and the content of genistin is more than or equal to 80%. The content of genistin is improved, and the soy isoflavone with high content of genistin has exact curative effects of improving the development of female secondary sex characteristics, preventing and curing climacteric syndromes, preventing female senile dementia and height atrophy, preventing and curing cancers related to estrogen secretion and the like. Therefore, the misgiving of consumer groups on the medical healthcare function of soy isoflavone is eliminated, and the production method disclosed by the invention can play an active promotion effect on the further development of the soy isoflavone industry.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a production method of soybean isoflavones with high genistin content. Background technique [0002] In recent years, soybean isoflavones have been widely used as natural phytoestrogens at home and abroad to improve the development of female secondary sexual characteristics, prevent menopausal syndrome, prevent female Alzheimer's disease and height atrophy, and prevent and treat cancers related to estrogen secretion. However, after oral intake of commercially available soy isoflavones by people, it is often found that the drug effect is not obvious, or even ineffective. [0003] At present, among soybean isoflavones found in soybean cotyledons, there are at least 12 isomers recognized by the field. figure 1 The high-performance liquid chromatogram of the content of various soybean isoflavone isomers in the cotyledons of soybeans in Jilin real estate tested by the inventor of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48
Inventor 李荣和徐国庆高长城李玉馨雷海容刘雷刘辉许伟良梁洪祥宛淑芳周书华
Owner CHANGCHUN UNIV
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