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Application of lambda-carrageenan oligosaccharide to preparation of anti-rabies virus drug

A technology for carrageenan oligosaccharide and rabies virus, which is applied in the directions of antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem of no anti-rabies virus, etc., and achieve the effect of long effective action time.

Active Publication Date: 2015-04-29
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

λ-carrageenan oligosaccharide has various biological activities such as anticoagulation, antivirus (such as herpes virus), antitumor, etc. However, there is no relevant literature report on antirabies virus at present

Method used

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  • Application of lambda-carrageenan oligosaccharide to preparation of anti-rabies virus drug
  • Application of lambda-carrageenan oligosaccharide to preparation of anti-rabies virus drug
  • Application of lambda-carrageenan oligosaccharide to preparation of anti-rabies virus drug

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Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with examples.

[0021] 1 Materials and methods

[0022] 1.1 Materials

[0023] 1.1.1 Viruses

[0024] Rabies virus laboratory fixed strains SAD-L16, CVS-B2c, rabies virus wild Anhui strain DRV-AH08 (canine rabies virus) are all preserved by the State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University.

[0025] 1.1.2 Marine oligosaccharides

[0026] The λ-carrageenan oligosaccharide was provided by Ocean University of China, with a molecular weight of about 4,000 Da and a maximum water solubility of 50 mg / mL. with DMEM or ddH respectively 2 O was dissolved into a 5mg / mL solution, sterilized by filtration through a 0.22μm microporous filter, and stored at 4°C after aliquoting. Cell growth solution, maintenance solution or ddH was used for experiments. 2 O was diluted to the desired concentration.

[0027] 1.1.3 Cells and monoclonal antibodies

[0028] SK-N-SH cells (h...

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Abstract

The invention discloses an application of lambda-carrageenan oligosaccharide to preparation of an anti-rabies virus drug. Experiments prove that the lambda-carrageenan oligosaccharide is low in cytotoxicity so that rabies viruses of various strains can be inhibited on various types of cells and inhibition effect is provided to each stage of the rabies viruses invading the cells; and particularly in the stage after virus adsorption, namely, 12 hours after the cells are infected by the viruses, the lambda-carrageenan oligosaccharide is still capable of remarkably decreasing the rabies virus amount in the cells. According to above discovery, the lambda-carrageenan oligosaccharide can be used as a low-cost effective wound treating agent to replace the anti-rabies virus immune globulin, and has the advantages of decreasing the viruses at the wound part to the greatest extent and reducing the rabies morbidity.

Description

technical field [0001] The invention relates to a new drug application of λ-carrageenan oligosaccharide, especially a new application in preparing anti-rabies virus drugs. Background technique [0002] Rabies is one of the oldest infectious diseases and is a zoonotic disease caused by Rabies virus (RABV). Rabies is by far the acute infectious disease with the highest human case fatality rate, once onset, the case fatality rate is almost 100%. According to the report of the World Health Organization (WHO), about 55,000 people die from rabies every year in the world, most of which are distributed in Africa and Asia. In recent years, an average of about 3,000 people died of rabies each year in my country, most of which occurred in vast rural areas or urban-rural fringe areas, and were mainly infected by bites of venomous animals. RABV enters the bite site with saliva, enters the nearest nerve fiber after a brief incubation in the muscle, and gradually migrates to the brain. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737A61P31/14
Inventor 赵凌傅振芳罗召晨周明管华诗王伟
Owner HUAZHONG AGRI UNIV
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