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Application of whey protein and reducing oligosaccharide Maillard product to microcapsule wall materials and embedded probiotics

A technology of microcapsule wall material and polysaccharide Maillard, which is applied in the fields of application, lactobacillus, and dairy products, can solve the problems of poor protection ability of a single wall material, weak fixed-point release ability, and insufficient nutrition of probiotics, and achieve Enhanced protection ability, convenient operation, and uniform particle size

Inactive Publication Date: 2015-04-29
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although the currently used wall materials and embedding techniques and methods have achieved a high embedding rate and good protection, they have poor protection ability of a single wall material, high cost of mixed wall materials, complicated operation, and poor fixed-point release ability. Strong, deficiencies such as insufficient nutrition of probiotics after release

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0047]Prepare the concentrated whey protein into a 10% (w / v) solution, add 1 / 4 of the protein amount of isomaltooligosaccharide, adjust the pH to 7.6 with 10M NaOH and 1M HCl, and put it in the refrigerator at 4°C overnight to make it Fully hydrated. Keep at 85°C for 3 hours to partially graft whey protein and isomaltooligosaccharide, and the reaction solution is the wall material solution. Cool to room temperature and store at 4°C for later use.

[0048] Prepare the core material solution, isolate and purify Lactobacillus rhamnosus LGG, and activate two generations, take the culture solution and centrifuge at 5000r / min for 5min at 4°C, take the sludge, dissolve it in normal saline, and make the bacterial concentration reach 10 9 About CFU / g.

[0049] To prepare a mixed emulsion, take soybean oil and span-80 and mix them uniformly at a volume ratio of 10:1. Heat to 40°C. The magnetic stirring speed is 700r / min.

[0050] Mix the wall material solution and the bacterial sus...

example 2

[0055] Prepare the concentrated whey protein into a 10% (w / v) solution, add 1 / 2 of the protein amount of isomaltooligosaccharide, adjust the pH to 7.6 with 10M NaOH and 1M HCl, and put it in the refrigerator at 4°C overnight to make it Fully hydrated. Keep at 85°C for 3 hours to partially graft whey protein and isomaltooligosaccharide, and the reaction solution is the wall material solution. Cool to room temperature and store at 4°C for later use.

[0056] Prepare the core material solution, isolate and purify Lactobacillus rhamnosus LGG, and activate two generations, take the culture solution and centrifuge at 5000r / min for 5min at 4°C, take the sludge, dissolve it in normal saline, and make the bacterial concentration reach 10 9 About CFU / g.

[0057] The wall material solution and bacterial suspension were mixed at a volume ratio of 10:1, and 4.0 g / L gluconolactone (GDL) was added as a coagulant. Stir quickly. Add it into soybean oil with magnetic stirring at 40°C, and s...

example 3

[0063] Prepare the concentrated whey protein into a 10% (w / v) solution, add 1 / 4 of the protein amount of isomaltooligosaccharide, adjust the pH to 7.6 with 10M NaOH and 1M HCl, and put it in the refrigerator at 4°C overnight to make it Fully hydrated. Keep at 85°C for 3 hours to partially graft whey protein and isomaltooligosaccharide, and the reaction solution is the wall material solution. Cool to room temperature and store at 4°C for later use.

[0064] Prepare the core material solution, isolate and purify Lactobacillus rhamnosus LGG, and activate two generations, take the culture solution and centrifuge at 5000r / min for 5min at 4°C, take the sludge, dissolve it in normal saline, and make the bacterial concentration reach 10 10 About CFU / g.

[0065] The microcapsules were produced by spray drying method, the inlet air temperature was 150°C, and the feed flow rate was 20.00mL / min to obtain dry microcapsules. The following indicators were detected:

[0066] The content o...

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PUM

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Abstract

The invention takes protein and a reducing oligosaccharide Maillard product as microcapsule wall materials and applies the protein and the reducing oligosaccharide Maillard product to the production of probiotic microcapsules. A wall material solution is prepared by reaction of following components: the whey protein and isomaltooligosacharide are mixed according to the mass ratio ranging from (1:1) to (10:1) to form a solution, heating treatment is carried out at a constant temperature of 85-95 DEG C for 2-4 hours, Maillard reaction is carried out to generate grafts with different contents, which are used as a wall material solution. The concentrated probiotics are used as a core material, and an endogenesis emulsification gel method and a spraying and drying method are used for producing the probiotic microcapsules. The microcapsule wall materials comprise the milk-based protein and the prebiotics, have nutritional and functional properties and have a wide application range. A Maillard product is used as a wall material structure and is dense, so that the protection capability is enhanced; the fixed point releasing capability is enhanced in the presence of intestinal canal enzymes and intestinal canal microorganisms; and after the releasing, the microcapsule wall materials can provide nutrients for the probiotics. The prepared microcapsules are used as function factors, have a good market prospect and can be used for medicine, food and feed industries.

Description

Technical field [0001] The present invention is the field of micro -capsule technology. Specifically, it is the application of protein and reducing hypocruster glycogen. Background technique [0002] Probiotics are defined by the Food and Agricultural Committee (FAO) and World Health Organization (WHO) as a type of microorganisms that can be beneficial to the human body in the human intestine.How to protect probiotics under the condition of human digestive tract to maintain vitality to achieve the role of probe in the specified location is a problem to solve the food industry.Therefore, studying how to maintain a high number of probiotics transmission and release the target position and the release of colonization after the release is the main problem. [0003] At present, more methods are used to protect probiotics with microcapsules.Commonly used single wall materials include sodium algina, chitosan, other polysaccharides (vascus, cellulose derivatives, transgender starch, etc....

Claims

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Application Information

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IPC IPC(8): A23L1/00A23L1/30A23C9/123A23C19/032A23G9/36A23K1/00
CPCA23C9/123A23C19/032A23G9/363A23V2002/00A23V2400/125A23V2400/175A23V2400/169A23V2250/54252A23V2250/6412A23V2200/3204
Inventor 李晓东朱永明李东花杜玲玲曲佳琳王春潮马赛荣庄建鹏
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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