A method for improving the anther culture efficiency of common wild rice hybrid progeny
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A culture method and technology of culture efficiency, applied in the field of plant tissue culture, can solve the problems of low anther culture efficiency, achieve the effect of speeding up the process of rice breeding, improving the rate of callus induction and seedling formation, and improving the efficiency of breeding
Inactive Publication Date: 2016-03-16
云南省农业科学院生物技术与种质资源研究所
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[0008] The object of the present invention is to overcome the problem of low anther culture efficiency in the prior art, and provide a method for improving the anther culture efficiency of common wild rice hybrid progeny, the specific steps are as follows:
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Embodiment 1
[0019] Example 1: Suspension Culture of Anthers of Common Wild Rice Japonica Hybrid Progeny in Yuanjiang, Yunnan
[0020] In this example, the japonica hybrid progeny F1-24 of Yuanjiang common wild rice in Yunnan was used as the test material, and haploid plants were obtained by the method of the present invention. The implementation process is as follows:
[0021] 1) Selection of explants: Take the young ears in the field where the dew is not dry in the morning on a sunny day, select the rice ears with a distance of 5 cm between the flag leaf ring and the next leaf ring, the ears are not broken, keep two leaves and leaf sheaths, and use volume Wipe the leaves and leaf sheaths with 70% alcohol cotton balls, then wrap the young ears with plastic wrap according to different strains, and place them in a 6°C refrigerator for low-temperature pretreatment for 7 days. Select the branch stalk whose spikelet anther is in the mononuclear stage, that is: the anther is light yellow, and ...
Embodiment 2
[0029] Embodiment 2: Suspension culture of anthers of common wild rice indica type hybrid progeny in Yuanjiang, Yunnan
[0030] In this example, SP13 and SP15, the indica hybrid progenies of common wild rice in Yuanjiang, Yunnan, were used as test materials, and haploid plants were obtained by using the method of the present invention. The implementation process is basically the same as in Embodiment 1, the difference is that:
[0031] 1) During the selection of the explant, choose the distance between the flag leaf annulus and the next leaf annulus to be 7cm rice ears, and select the branches whose anthers of the spikelets are in the single-nucleus stage, that is: the anthers are light yellow, and the length of the stamens in the glume is long. Anther callus induction was carried out on rice ears close to 2 / 5 of the glume length.
[0032] 2) The induction time of anther callus in liquid suspension is 20 days.
[0033] 3) It takes 30 days for anther callus differentiated seedl...
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Abstract
The invention relates to a method for improving the culture efficiency of the anther of the filial generation of Oryza rufipogon, and belongs to the field of a plant tissue culture technology. The method for improving the culture efficiency of the anther of the filial generation of the Oryza rufipogon comprises the following steps: selecting an explant, sterilizing the explant, inducing an anther derived callus by use of fluid suspension, performing multiplication culture on the anther derived callus, performing differentiation culture on the anther derived callus, performing rooting culture on a differentiated seedling (anther cultured seedling), hardening the seedling and transplanting. According to the method, the anther is inoculated in a fluid nutrient medium to be subjected to suspension culture, the callus induction rate is improved, the callus is transferred onto a multiplying medium to be subjected to the multiplication culture, the quality of the callus is improved and the quantity of the callus is increased. The method has the advantages that the callus induction rate and the seedling survival rate in the anther culture of the filial generation of the Oryza rufipogon are improved greatly, the rice breeding process is accelerated, the breeding efficiency of the good rice variety is improved, and the method can provide a powerful technical support for acceleration of breeding of rice varieties by use of good genes of the Oryza rufipogon.
Description
Technical field: [0001] The invention relates to a method for cultivating anthers of hybrid offspring of common wild rice, belonging to the technical field of plant tissue culture. Background technique: [0002] Wild rice is a general term for wild species in the genus Oryza excluding cultivated rice (O. sativa L.) and African cultivated rice (O. glaberrima Steud.). The common wild rice belongs to the A'A' genome, which is relatively close to the cultivated AA genome. Therefore, the common wild rice is an excellent resource for distant hybrid breeding of rice. [0003] The traits of common wild rice and the offspring of cultivated distant hybrid breeding are wildly separated. To stabilize the traits, multiple generations of repeated backcrossing, selfing and selection are required. Even in Hainan, where three seasons are planted a year, it takes at least 4-5 years for breeding. The use of rice pollen culture can speed up the homozygosity of target traits and shorten the bre...
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