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Multi-gene high rice blast resistance material breeding method adopting marker assisted selection

A molecular marker-assisted, rice blast resistance gene technology, applied in plant genetic improvement, botanical equipment and methods, applications, etc., can solve problems such as heavy workload, difficult identification, blindness, etc., and achieve the effect of improving efficiency

Inactive Publication Date: 2015-04-29
天津天隆农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional pedigree method selects the best breeding varieties from the separated offspring through the combination of two parents. The disease resistance phenotype depends on natural identification in the field or artificial inoculation identification, which is difficult to identify and has a low accuracy rate.
Because they do not know the disease-resistant genotype of the parents, the breeders are very blind in the selection of the parents, usually mass matching, massive selection of offspring, heavy workload and low breeding efficiency

Method used

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  • Multi-gene high rice blast resistance material breeding method adopting marker assisted selection
  • Multi-gene high rice blast resistance material breeding method adopting marker assisted selection
  • Multi-gene high rice blast resistance material breeding method adopting marker assisted selection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Identification and screening of the offspring population constructed by crossing the resistant parent P1 containing the rice blast resistance gene Pita and the susceptible parent Liaoxing No. 1

[0027] The resistant parent P1 containing the rice blast resistance gene Pita was crossed with the susceptible parent Liaoxing 1, and then selfed, and then the selfed F2 generation was screened for traits and rice blast resistance gene Pita, and the plant height, growth period, 50 F2 single plants, which are similar to Liaoxing 1 in high yield and contain the disease resistance gene Pita; then backcrossed with the susceptible parent Liaoxing 1 for the first time, and self-crossed to obtain BC 1 f 2 , and then screened for traits and disease-resistant gene Pita, and selected BC with excellent traits and disease-resistant gene Pita 1 f 2 50 plants per plant; then carry out a second backcross with the susceptible parent Liaoxing 1, and self-cross to obtain BC 2 f 2,...

Embodiment 2

[0028] Example 2: Identification and screening of resistant parent P2 containing rice blast resistance gene Pi5 crossed with susceptible parent Liaoxing No. 1 to construct a resistant and susceptible population

[0029] The resistant parent P2 containing the rice blast resistance gene Pi5 was crossed with the susceptible parent Liaoxing 1, and then selfed, and then the selfed F2 generation was screened for traits and rice blast resistance gene Pi5, and the plant height, growth period, 50 F2 single plants, which are similar in yield and yield to Liaoxing 1 and contain the disease resistance gene Pi5; then backcrossed with the susceptible parent Liaoxing 1 for the first time, and self-crossed to obtain BC 1 f 2 , and then screened for traits and disease-resistant gene Pita, and selected BC with excellent traits and disease-resistant gene Pi5 1 f 2 50 plants per plant; then carry out a second backcross with the susceptible parent Liaoxing 1, and self-cross to obtain BC 2 f 2 ...

Embodiment 3

[0030] Example 3: Gene aggregation of the offspring BC2F4 strain containing the rice blast resistance gene Pita and the offspring BC2F4 strain containing the rice blast resistance gene Pi5

[0031] Use the BC2F4 generation of Pita individual plants containing the disease resistance gene to cross with the disease resistance gene pi5 individual plants, self-cross to obtain F2, and use molecular markers A1, A2, A3, A4, B1, B2 to screen for disease resistance genes Pita, pi5 , and combined with the identification of disease resistance in the field, select a single plant that contains two disease resistance genes and performs well in the field for selfing plus generation. A new disease-resistant variety containing Pita and pi5 disease-resistant genes with pure and stable performance in the field was obtained by self-crossing and adding three generations.

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Abstract

The invention relates to a multi-gene high rice blast resistance material breeding method adopting marker assisted selection, and belongs to the field of rice breeding. According to the method, specific molecular marker assisted selection of rice blast resistance genes pita and pi5 is adopted, field resistance identification is combined, and accordingly, a new disease-resistant variety can be bred effectively. The method comprises implementation modes as follows: 1, parents P1 and P2 containing the rice blast resistance genes pita and pi5 respectively are hybridized with a susceptible parent Liaoxing 1 to establish a resistant and susceptible offspring group; 2, extraction of DNA of single resistant and susceptible offspring, PCR (polymerase chain reaction) amplification and electrophoretic analysis of different markers are performed; 3, the parents P1 and P2, the susceptible variety Liaoxing 1 and the single plant of the hybridized offspring group are planted in a rice blast high incidence area Donggang, Dandong, and leaf blast and spike and stem blast resistance identification is performed at a seedling stage and a maturation stage respectively. In combination of the resistance gene marker selection, the new multi-gene rice blast resistance variety containing pita and pi5 is directionally improved and cultivated.

Description

technical field [0001] The invention relates to a breeding method for molecular marker-assisted selection of multi-gene high rice blast resistance materials, especially the use of specific molecular marker-assisted selection to polymerize rice materials containing multiple rice blast resistance genes, combining field phenotypic traits and disease resistance Sex identification, more clear and efficient breeding goals, belonging to the field of rice breeding. Background technique [0002] Rice blast is a disease of rice caused by Magnaporthe grisea, and its distribution range is very wide. According to statistics, it occurs in more than 80 countries in the world, and it can cause a 10%-20% reduction in production in general epidemic years, and it can reach 40%-50% in severe cases. Since the 1990s, the annual occurrence area of ​​rice blast in my country has been 3.8 million ha 2 Above, hundreds of millions of kilograms of rice are lost every year. With the simplification an...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 刘欣亓娜曲丽君朱崴刘桂林李志彬
Owner 天津天隆农业科技有限公司
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