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Hybridoma cell strain secreting monoclonal antibody against Iris yellow spot virus and application of monoclonal antibody

A hybridoma cell line, iris macular virus technology, applied in antiviral immunoglobulin, analytical materials, biochemical equipment and methods, etc., can solve the problem of undeveloped monoclonal antibodies, unsuitable for large-scale field sample testing, etc. question

Active Publication Date: 2015-04-15
ZHEJIANG UNIV +1
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Problems solved by technology

All of these methods have their limitations and are not suitable for the detection of large quantities of field samples
Serological methods are suitable for batch detection of field samples, but must rely on specific virus monoclonal antibodies
At present, no specific monoclonal antibody against this virus has been developed at home and abroad.

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  • Hybridoma cell strain secreting monoclonal antibody against Iris yellow spot virus and application of monoclonal antibody
  • Hybridoma cell strain secreting monoclonal antibody against Iris yellow spot virus and application of monoclonal antibody

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Embodiment Construction

[0014] Hybridoma cell line 12C10 secreting monoclonal antibody against iris macula virus, which can secrete specific monoclonal antibody against iris macula virus, hybridoma cell line 12C10 was preserved in China Committee for Culture Collection of Microorganisms on July 3, 2014 General Microbiology Center, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Zip Code: 100101, the preservation number is CGMCC No.9336, and the classification is named: secreting anti-Iris macula virus (IYSV) monoclonal antibody hybridoma cells;

[0015] Ascites ELISA titer of anti-iris macular virus monoclonal antibody reaches 10 -7 , the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can have a specific immune binding reaction with the capsid protein of iris macula virus, and can detect the iris macula virus in the thrips, the transmission medium;

[0016] Monoclonal antibodies against iris yellow spot virus are specific only for iris yellow spot viru...

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Abstract

The invention discloses a hybridoma cell strain secreting monoclonal antibody against Iris yellow spot virus and application of the monoclonal antibody. Iris yellow spot virus particles purified by a differential centrifugation method are used as an antigen to immunize BALB / c mice; through cell fusion, screening and cloning, one strain of hybridoma cell strain 12C10 capable of stable passage and secreting anti-Iris yellow spot virus monoclonal antibodies is obtained; and the strain has a preservation number CGMCC No.9336. The monoclonal antibody secreted from the hybridoma cell strain has ascites indirect ELISA titer of 10 <-7>, and the antibody type and subtype are IgG1, kappa chain. The monoclonal antibody specifically and sensitively detect Iris yellow spot virus. The reaction with ZYMV. The monoclonal antibody secreted from 12C10 only has specific reaction with Iris yellow spot virus, but does not react with tomato spotted wilt virus, turnip mosaic virus and cucumber mosaic virus. The hybridoma cell strain 1C5 and monoclonal antibody secreted by the cell strain provide technical and material support for the diagnosis, inspection and quarantine, and scientific prevention and control of the virus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting an anti-iris yellow spot virus (IYSV) monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Iris yellow spot virus (IYSV) is a Tospovirus belonging to the family Bunyaviridae. Since the 1980s, with the improvement of plant virus research methods and the rapid development of molecular biology techniques, many new viruses in the genus Tospovirus have been identified and named. IYSV was first discovered on Iris in the Netherlands in 1992. According to literature reports, IYSV has a narrow host range, but it can infect monocots. When the iris is infested, chlorotic spots initially appear, which develop into yellow necrotic spots as the disease progresses. Depending on the cultivar, 50% to 95% of plants can be infected. In addition, IYSV can also naturally infect vegetable crops such as onions and leeks. L...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569C12R1/91
Inventor 吴建祥于翠周雪平李娜
Owner ZHEJIANG UNIV
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