A kind of determination method of chlorantraniliprole residues in vegetables and fruits

Abstract

The invention discloses a method for measuring the residual amount of chlorantraniliprole in vegetables and fruits. The method disclosed by the invention comprises the following steps: homogenizing and extracting chlorantraniliprole residual in a sample by using acetonitrile or an acetonitrile solution containing 1% of acetic acid, dispersing and purifying an extraction solution through ethanediamine-N-propoxysilane (PSA) and an octadecylsilan bonded phase (C18), detecting through gas chromatography-electron impact ion source-mass spectrum (GC-EI-MS), establishing a corrected standard curve by using a vehicle solution free from a pesticide to be measured, and quantifying by using an external standard method. According to the invention, the average recovery rate is 89.6%-103.5%; the average relative standard deviation (RSD) is 4.4%-7.7%; the detection limit is less than 0.62 microgram / kg; and the method disclosed by the invention has the advantages of being simple, convenient and rapid to operate, high in sensitivity, good in repeatability and accurate in qualitative and quantitative performance, and can satisfy the technological requirements on safety detection of corresponding products in the countries, such as America, Japan, European union and Canada, and provide strong technical support for sound development of food safety and foreign export trade in China.

Description

technical field [0001] The invention relates to a method for determining the residual amount of chlorantraniliprole in vegetables and fruits, more specifically, it uses gas chromatography-electron bombardment ion source-mass spectrometry (GC-EI-MS) to qualitatively and quantitatively determine the residues in vegetables and fruits. The method for the content of chlorantraniliprole belongs to the technical field of determination of pesticide residues. Background technique [0002] Chlorantraniliprole (Chlorantraniliprole), trade name Kang Kuan, is a new type of o-formamidobenzamide insecticide developed by DuPont. Mothidae, Fruit Mothidae, Leaf Rolleridae, Melydidae, Plutella Plutella, Graciidae, Thymothidae, etc. all have very good control effects, and can also control Coleoptera Weevilidae and Chromophyidae; Muscidae; Bemisia tabaci and other non-Lepidoptera pests. The chemical name is: 3-bromo-N-[4-chloro-2-methyl-6-[(methylcarbamoyl)benzene]-1-(3-chloropyridin-2-yl)-1H-...

AI Technical Summary

Problems solved by technology

[0008] In recent years, there have been many studies on the detection methods of chlorantraniliprole residues in various food and agricultural products. The detection methods reported all use liquid chromatography (LC) or liquid chromatography tandem mass spectrometry (LC-MS / MS) to determine the residues of vegetables and chlorantraniliprole. The detection method of cyantraniliprole residues in fruits, using LC-MS / MS to determine pesticide residues in food and agricultural products has the advantages of fast, simple, and high sensitivity, but due to its high price, many testing institutions, enterprises or research institutes have not equipped The number of instruments or configurations is small, because when different compounds are detected by LC-MS / MS, different mobile phases or chromatographic columns need to be used, which requires constant replacement of chromatographic columns and mobile phases and takes a long time to balance the system , which to some extent restricts the application of LC-MS / MS

Gas chromatography-mass spectrometry (GC-EI-MS) equipped with electron bombardment ionization source has great advantages in analyzing pesticide residues in food and agricultural products. Since electron bombardment ionization source mass spectrometry is a universal detector, it can realize multi-residue analysis of hundreds of pesticides. It can be qualitative and quantitative at the same time, and the price is moderate. Therefore, various testing institutions and enterprises are equipped with gas chromatography-electron bombardment ion source-mass spectrometer (GC-EI-MS) to detect pesticide residues in food and agricultural products, but so far no See the report of the GC-EI-MS detection method of chlorantraniliprole residue in vegetables and fruits, therefore, establish gas chromatography-electron bombardment ion source-mass spectrometry (GC-EI-MS) qualitative and quantitative analysis in vegetables and fruits The detection method of chlorantraniliprole residue is of great significance

Images