QAMS for detecting content of organic acids in sowthistle-leaf ixeris seedling injection

Abstract

The invention relates to the field of analysis of traditional Chinese medicine components, and relates to QAMS for detecting the content of organic acids in sowthistle-leaf ixeris seedling injection. The QAMS disclosed by the invention comprises the following steps: (1), setting chromatographic conditions; (2), preparing a reference solution; (3), preparing a test solution; and (4), calculating correction factors. Relative correction factors of chicoric acid, mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid are calculated by taking chicoric acid as the internal reference compound, and used as constants for detecting the content; correction factors are calculated by taking effective active components in the sowthistle-leaf ixeris seedling injection and high-content chicoric acid as internal reference compounds; the content of mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid in the sowthistle-leaf ixeris seedling injection are calculated; the content of the four organic acids in the injection can be detected simultaneously; therefore, the quality of the sowthistle-leaf ixeris seedling injection in industrial production can be monitored scientifically, sensitively and rapidly; and simultaneously, the problem that the quality of the sowthistle-leaf ixeris seedling injection cannot be controlled objectively and reasonably because of shortage of reference substances can be solved.

Description

technical field [0001] The invention relates to the field of component analysis of traditional Chinese medicines, that is, the one-measurement-multiple-evaluation method is used to determine the content of organic acids in Kudiezi injection. Background technique [0002] In the prior art, Kudiezi has another name called Gypsophila gypsophila, and it is mainly distributed in Northeast my country and Inner Mongolia. Grass, cold in nature, bitter and pungent in taste, has the effects of clearing heat and detoxifying, draining pus and relieving pain [1] . Kudiezi (Diemailing) Injection is an intravenous injection made from Kudiezi extracted and refined, which has the functions of promoting blood circulation, removing blood stasis and improving microcirculation [2] . The injection has been on the market for many years, and its curative effect is definite. Experimental studies have shown that the chemical composition of Kudiezi injection is relatively complex, and the main acti...

AI Technical Summary

Problems solved by technology

Using cichoric acid, which is an effective active ingredient in Kudiezi injection with a relatively high content, as an internal standard, the correction factor is calculated to calculate the contents of monocaffeoyl tartaric acid, chlorogenic acid, and caffeic acid in Dietzi injection, which can simultaneously determine the injection The content of four organic acid components in the solution can realize scientific, sensitive and rapid monitoring of the quality of Kudiezi injection in industrial production, and at the same time solve the problem that the quality of Kudiezi injection cannot be objectively and reasonably controlled due to the lack of reference substances

Images