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A kind of detection method of the residues of methapon in ripe oranges

A detection method, the technology of methapone, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, no special method for ripe oranges, and inability to detect oranges, so as to achieve good extraction effect and simple and easy-to-operate detection method , good separation effect

Active Publication Date: 2016-05-25
SUZHOU APP SCI ACAD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the commonly used method is complicated to operate, and there is no special method for ripe oranges. The residual amount of methadion in the orange peel and pulp should be different, and the orange cannot be detected as a whole.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the present embodiment, a kind of detection method of methadion residue in ripe orange comprises the following steps:

[0023] (1) Sample processing: Divide the ripe oranges into two parts, orange peel and pulp, cut the orange peel into pieces, and fry the pulp into juice with a juicer for later use;

[0024] (2) Extraction: Take 50g of orange peel and 100g of pulp and place them in conical flasks respectively, add 250ml and 500ml of acetonitrile-water mixture, shake and extract for 50min at a temperature of 40°C; filter to obtain clear liquid; Concentrate the supernatant to less than 10ml, and re-concentrate to 10ml with water to obtain orange peel samples and pulp samples for later use;

[0025] (3) Small column separation: put neutral aluminum oxide powder in a muffle furnace, activate at 680°C for 4 hours, load the column, first use 20ml of acetonitrile to pass through the column, and load the sample, first use 10ml of acetonitrile with a volume ratio of 1:1 A m...

Embodiment 2

[0030] In the present embodiment, a kind of detection method of methadion residue in ripe orange comprises the following steps:

[0031] (1) Sample processing: Divide the ripe oranges into two parts, orange peel and pulp, cut the orange peel into pieces, and fry the pulp into juice with a juicer for later use;

[0032] (2) Extraction: Take 50g of orange peel and 100g of pulp and place them in conical flasks respectively, add 250ml and 500ml of acetonitrile-water mixture, shake and extract at a temperature of 35°C for 70min; Concentrate the supernatant to less than 10ml, and re-concentrate to 10ml with water to obtain orange peel samples and pulp samples for later use;

[0033] (3) Small column separation: put neutral aluminum oxide powder in a muffle furnace, activate at 680°C for 4 hours, load the column, first use 20ml of acetonitrile to pass through the column, and load the sample, first use 10ml of acetonitrile with a volume ratio of 1:1 A mixture of acetonitrile and wate...

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Abstract

The invention discloses a method for detecting residual methidathion in a ripe orange. The method for detecting residual methidathion in the ripe orange comprises the following steps: (1) dividing a ripe orange into an orange peel part and a pulp part, cutting the orange peel into pieces, and squeezing pulp with a juice extractor to obtain juice for later use; (2) respectively putting the orange peel and pulp into conical flasks, respectively adding acetonitrile water mixed liquor, and carrying out vibrating extraction for 50-70 minutes; respectively carrying out suction filtration to obtain clear liquids; concentrating the clear liquids respectively, and realizing constant volume being 10ml again by adopting water, so as to obtain an orange peel sample and a pulp sample for later use; (3) putting neutral aluminium oxide powder into a muffle furnace, activating for 4 hours at the temperature of 680 DEG C, packing, firstly passing a column with 20ml acetonitrile, loading the samples, firstly activating a small column with 10ml mixed liquor of acetonitrile and water in a volume ratio of 1:1, then leaching with 20ml acetonitrile, collecting leacheate, carrying out rotary evaporation until volume of the leacheate is 1ml, blow-drying with nitrogen, realizing constant volume being 5ml by adopting acetone, and making preparation for detection; (4) detecting by adopting a gas chromatography.

Description

technical field [0001] The invention relates to a method for detecting methadion residues in ripe oranges, and belongs to the technical field of pesticide residue detection. Background technique [0002] Methidathion is an organophosphorus non-systemic insecticide with certain acaricidal activity. It is mainly used to control a variety of piercing and sucking pests and leaf-eating pests, especially scale insects. It is basically harmless . The pure product is a colorless crystal with a melting point of 34~40°C. At 20°C, its solubility in water is 250mg / L. The agent is stable in neutral and weakly acidic media, but unstable in strong acid and alkaline media. In addition, metapon is highly toxic to humans and animals. The acute oral toxicity LD50 of the original drug to male rats is 26 mg / kg, and that of female rats is 43.8 mg / kg; It is 200mg / kg; it has no irritating effect on the eyes of rabbits, but is slightly irritating to the skin; it is highly toxic to fish, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 徐晓华金艳华
Owner SUZHOU APP SCI ACAD CO LTD
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