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Method for detecting residual methidathion in ripe orange

A detection method and technology for killing phosphorus, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of no special method for ripe oranges, complicated operations, and inability to detect oranges, and achieve a simple and easy-to-operate detection method and good extraction effect. , good separation effect

Active Publication Date: 2015-04-08
SUZHOU APP SCI ACAD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the commonly used method is complicated to operate, and there is no special method for ripe oranges. The residual amount of methadion in the orange peel and pulp should be different, and the orange cannot be detected as a whole.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this embodiment, a method for detecting chlorhexidine residues in ripe oranges includes the following steps:

[0023] (1) Sample processing: Divide the ripe orange into two parts: orange peel and pulp, cut the orange peel into pieces, and fry the pulp into juice with a juicer, and set aside;

[0024] (2) Extraction: Take 50g of orange peel and 100g of fruit pulp respectively in an Erlenmeyer flask, add 250ml and 500ml of acetonitrile water mixture respectively, shake and extract for 50min at a temperature of 40℃; filter to obtain a clear liquid; Concentrate the clear liquid to less than 10ml, and re-set the volume to 10ml with water to obtain orange peel samples and pulp samples for later use;

[0025] (3) Small column separation: Place the neutral aluminum oxide powder in a muffle furnace, activate at 680°C for 4 hours, load the column, first pass the column with 20ml acetonitrile, load the sample, first use 10ml volume ratio 1:1 Activate the cartridge with a mixture of a...

Embodiment 2

[0030] In this embodiment, a method for detecting chlorhexidine residues in ripe oranges includes the following steps:

[0031] (1) Sample processing: Divide the ripe orange into two parts: orange peel and pulp, cut the orange peel into pieces, and fry the pulp into juice with a juicer, and set aside;

[0032] (2) Extraction: Take 50g of orange peel and 100g of fruit pulp respectively in an Erlenmeyer flask, add 250ml and 500ml of acetonitrile water mixture respectively, shake and extract for 70min at a temperature of 35°C; filter to obtain a clear liquid; Concentrate the clear liquid to less than 10ml, and re-set the volume to 10ml with water to obtain orange peel samples and pulp samples for later use;

[0033] (3) Small column separation: Place the neutral aluminum oxide powder in a muffle furnace, activate at 680°C for 4 hours, load the column, first pass the column with 20ml acetonitrile, load the sample, first use 10ml volume ratio 1:1 Activate the cartridge with a mixture of ...

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Abstract

The invention discloses a method for detecting residual methidathion in a ripe orange. The method for detecting residual methidathion in the ripe orange comprises the following steps: (1) dividing a ripe orange into an orange peel part and a pulp part, cutting the orange peel into pieces, and squeezing pulp with a juice extractor to obtain juice for later use; (2) respectively putting the orange peel and pulp into conical flasks, respectively adding acetonitrile water mixed liquor, and carrying out vibrating extraction for 50-70 minutes; respectively carrying out suction filtration to obtain clear liquids; concentrating the clear liquids respectively, and realizing constant volume being 10ml again by adopting water, so as to obtain an orange peel sample and a pulp sample for later use; (3) putting neutral aluminium oxide powder into a muffle furnace, activating for 4 hours at the temperature of 680 DEG C, packing, firstly passing a column with 20ml acetonitrile, loading the samples, firstly activating a small column with 10ml mixed liquor of acetonitrile and water in a volume ratio of 1:1, then leaching with 20ml acetonitrile, collecting leacheate, carrying out rotary evaporation until volume of the leacheate is 1ml, blow-drying with nitrogen, realizing constant volume being 5ml by adopting acetone, and making preparation for detection; (4) detecting by adopting a gas chromatography.

Description

Technical field [0001] The invention relates to a method for detecting the residues of pyridoxine in ripe oranges, and belongs to the technical field of pesticide residue detection. Background technique [0002] Methidathion is an organophosphate non-systemic insecticide with a certain acaricidal activity. It is mainly used to control a variety of piercing and leaf-eating pests, especially scale insects. It is basically free of pesticide damage . The pure product is a colorless crystal with a melting point of 34-40°C. At 20°C, its solubility in water is 250 mg / L. The agent is stable in neutral and weak acid media, but unstable in strong acid and alkaline media. In addition, acetaminophen is highly toxic to humans and animals. The original drug has an acute oral toxicity LD50 of 26 mg / kg for male rats and 43.8 mg / kg for female rats; acute dermal toxicity LD50 for rats is 1 546 mg / kg It is 200mg / kg for rabbits; it has no irritation to rabbit eyes, but has mild skin irritation; ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 徐晓华金艳华
Owner SUZHOU APP SCI ACAD CO LTD
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