Small-molecular novel-miR-1 and osteosarcoma medicine

Abstract

The invention provides a small-molecular novel-miR-1 with a base sequence of a sequence table SEQ.ID.No.1. Bioinformatics analysis on the small-molecular novel-miR-1 indicates that the small-molecular RNA is related to the osteosarcoma expressed potential target gene; meanwhile, a series of growth and propagation related cell biology experiments are conducted on the screened novel-miR-1 expressed osteosarcoma cells based on the bioinformatics analysis, and the result indicates that the period of the novel-miR-1 transfected osteosarcoma cells is sluggish, the proliferation of the osteosarcoma cells is inhibited, and the transfer ability and tumor forming ability of the tumor cells decline; the small-molecular novel-miR-1 is used as a medicine against osteosarcoma simply by transfecting the novel-miR-1 and intruding into the sick osteosarcoma tissue part; and after transfection, the cloning and proliferation of the osteosarcoma cells can be inhibited by changing the gene expression in the osteosarcoma cells.

Description

technical field [0001] The invention belongs to the technical field of small molecule RNA drugs, in particular to a small molecule novel-miR-1 and a drug for osteosarcoma. Background technique [0002] Osteosarcoma is the most common type of malignant bone tumor, which develops from mesenchymal cell lines. The rapid growth of the tumor is due to the direct or indirect formation of tumor bone-like tissue and bone tissue through the cartilage stage. After the pathological diagnosis of osteosarcoma, the traditional treatment methods are to start the early chemical or radiotherapy, and to remove the tumor tissue and so on. However, this method also has strong damage to normal bone tissue cells, and at the same time, tumor cells cannot recur. Therefore, more and more methods are available that tend to curative methods, such as total resection and immunotherapy. [0003] Among them, immunotherapy involves intravenous infusion of lymphocytes or the use of interferon and transfer ...

AI Technical Summary

Problems solved by technology

[0003] Among them, immunotherapy is the intravenous infusion of lymphocytes or the use of interferon and transfer factors; by inducing the immune expression of cells in the body, the matrix can produce autonomous inhibitory expression, but the curative effect is not yet certain

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