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Regulatory-cytokine-induced anti-apoptotic molecule (CIAPIN1) recombinant adenovirus and preparation method and use thereof

A technology of gene recombination and adenovirus is applied in the field of biogenetic engineering to achieve the effects of reducing damage, inhibiting growth, and improving development and application prospects

Inactive Publication Date: 2012-12-12
ARMY MEDICAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

So far, there have been no research reports on the regulatory CIAPIN1 gene recombinant adenovirus and its application in the field of glioma treatment at home and abroad

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  • Regulatory-cytokine-induced anti-apoptotic molecule (CIAPIN1) recombinant adenovirus and preparation method and use thereof
  • Regulatory-cytokine-induced anti-apoptotic molecule (CIAPIN1) recombinant adenovirus and preparation method and use thereof
  • Regulatory-cytokine-induced anti-apoptotic molecule (CIAPIN1) recombinant adenovirus and preparation method and use thereof

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Embodiment Construction

[0025] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0026] 1. Preparation of regulatory CIAPIN1 gene recombinant adenovirus

[0027] 1. Cloning of Survivin promoter

[0028] Genomic DNA of U251 glioma cells was extracted using a genomic DNA extraction kit, and the operation was performed according to the instructions of the kit. Using the obtained U251 cell genomic DNA as a template, F1: 5'-gc ctcgag gctcaagtgacaagcaagtgtttat-3' (SEQ ID No.1, the underlined part is the XhoⅠ restriction site) and R1: 5'-gc ccatgg gggctcacctggctgctc-3' (SEQ ID No.2, t...

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Abstract

The invention discloses regulatory-CIAPIN1 gene recombinant adenovirus and a preparation method and use thereof. The recombinant adenovirus is obtained by inserting a CIAPIN1 gene expression cassette into the genome of a replication-deficient type-5 adenovirus, wherein the CIAPIN1 gene expression cassette consists of a survivin promoter, a CIAPIN1 gene and a terminator; and the expression of the CIAPIN1 gene is regulated by the survivin promoter. The recombinant adenovirus has high selectivity and specificity; by regulating the expression of the CIAPIN1 gene through the tumor specific survivin, the CIAPIN1 gene can be selectively expressed in glioma cells to inhibit cell cloning and induce cell apoptosis; and thus, glioma cells can be accurately killed with less damage to normal tissue cells. In-vivo experiments indicate that the regulatory-CIAPIN1 gene recombinant adenovirus can inhibit the growth of glioma cells and prolong the average survival period of tumor bearing nude mice; therefore, the regulatory-CIAPIN1 gene recombinant adenovirus can be used for preparing medicines for treating glioma and has a good development and application prospect in the field of gene therapy of glioma.

Description

technical field [0001] The invention belongs to the field of biological genetic engineering, relates to a gene recombined adenovirus, in particular to a regulatory CIAPIN1 gene recombined adenovirus, and also relates to a preparation method and application of the recombined adenovirus. Background technique [0002] Glioma is an important disease that threatens human health, and research on new methods of treatment has always been the focus of medical and life science research. The biological treatment of glioma has been recognized by the world medical community as the fourth major treatment method after surgery, radiotherapy and chemotherapy, and gene therapy is one of the most active research fields in tumor biological treatment. The principle of gene therapy for glioma is to introduce the target gene into target cells using gene transfer technology, so that the target cells express the gene to obtain specific functions, and then execute or mediate the killing and inhibiti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/113C12N15/12C12N15/861A61K38/17A61P35/00C12R1/93
CPCY02A50/30
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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