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Isolated culture method of saussurea involucrata stem cells and application of saussurea involucrata stem cells in preparation of anti-senile product

A technology for separating and culturing stem cells, which is applied to the separation and culture of snow lotus stem cells and the application in the preparation of anti-aging products. Attack and damage reduction effects

Active Publication Date: 2015-04-08
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of animal-derived stem cells is controversial in many countries, not only has potential safety hazards, but also has many humane restrictions
Most of the plant stem cells Su Ri'an are totipotent and have the characteristics of stem cells. However, they have more homology with the human body. Therefore, the functions of plant stem cell extracts are not very clear at present, which hinders the corresponding application.

Method used

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  • Isolated culture method of saussurea involucrata stem cells and application of saussurea involucrata stem cells in preparation of anti-senile product
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  • Isolated culture method of saussurea involucrata stem cells and application of saussurea involucrata stem cells in preparation of anti-senile product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Preparation of Tianshan snow lotus material

[0047] The present invention gets the tender leaves of Snow Lotus of Tianshan Mountain, and the leaves are washed with running water for 2 hours; the surface is disinfected with 70% alcohol for 10 seconds, and rinsed with sterile water for 3 times; after 4, 6, 8, and 10 minutes are respectively disinfected with 0.1% liters of tribute solution, Rinse 3 times with sterile water to wash off the residual liquid on the surface of the plant to form different disinfection treatments; put the sterilized materials into a sterile petri dish, and dry the water with sterile filter paper for later use.

[0048] (2) Isolate Tianshan Saussurea stem cells from the material

[0049] Under sterile conditions, the sterilized young leaves of snow lotus are cut into a size of about 0.5cm*0.5cm, and inoculated on a solid medium. When inoculating, the back of the leaf is in contact with the medium, 1 piece per bottle. The culture temperature...

Embodiment 2

[0061] (1) Preparation of Tianshan snow lotus material

[0062] The present invention gets the young and tender leaves of Saussurea chinensis, and the leaves are rinsed with running water for 4 hours; surface-sterilized with 75% alcohol for 15 seconds, and rinsed with sterile water for 5 times; after 4, 6, 8, and 10 minutes were respectively disinfected with a concentration of 0.1% tribute solution, Rinse 5 times with sterile water to wash away the residual liquid on the surface of the plant to form different disinfection treatments; put the sterilized materials into a sterile petri dish, and dry the water with sterile filter paper for later use.

[0063] (2) Isolate Tianshan Saussurea stem cells from the material

[0064] Under sterile conditions, the sterilized young leaves of snow lotus are cut into a size of about 0.5cm*0.5cm, and inoculated on a solid medium. When inoculating, the back of the leaf is in contact with the medium, 1 piece per bottle. The culture temperatur...

Embodiment 3

[0076] (1) Preparation of Tianshan snow lotus material

[0077] The present invention gets the tender leaves of Snow Lotus of Tianshan, and the leaves are rinsed with running water for 3 hours; sterilized with 75% alcohol on the surface for 15 seconds, rinsed with sterile water for 4 times; after 4, 6, 8, and 10 minutes were respectively disinfected with 0.1% liters of tribute solution, Rinse 4 times with sterile water to wash away the residual liquid on the surface of the plant to form different disinfection treatments; put the sterilized materials into a sterile petri dish, and dry the water with sterile filter paper for later use.

[0078] (2) Isolate Tianshan Saussurea stem cells from the material

[0079] Under sterile conditions, the sterilized young leaves of snow lotus are cut into a size of about 0.5cm*0.5cm, and inoculated on a solid medium. When inoculating, the back of the leaf is in contact with the medium, 1 piece per bottle. The culture temperature is 27°C, th...

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Abstract

The invention discloses an isolated culture method of saussurea involucrata stem cells and an application of the saussurea involucrata stem cells in preparation of an anti-senile product. The method particularly comprises the following steps: taking tender leaves of saussurea involucrata, and sterilizing; firstly, separating the saussurea involucrata stem cells by virtue of a solid culture medium, and then carrying out subculture by virtue of a liquid culture medium, wherein the solid culture medium contains an inorganic salt, vitamin, amino acid, auxin, saccharides and activated carbon; and the liquid culture medium contains an inorganic salt, vitamin, amino acid, auxin, saccharides and an additive. The activity of SOD in the cells is improved by a human adipose-derived stem cell extract, thus the attack and the damage to the cells caused by oxygen radicals generated by ultraviolet radiant inducement are reduced; and the death rate of the cells is reduced, thus the effect of protecting the cells is achieved, and the anti-light aging efficacy is achieved.

Description

technical field [0001] The present invention relates to the application of plant stem cells in anti-aging products, in particular to a method for separating and culturing Saussurea oleifera stem cells and their application in preparing anti-aging products. Background technique [0002] Stem cells are seed cells that have not yet differentiated and developed, with unlimited self-renewal replication and differentiation potential, which is the basis for stem cells to be used in cosmetic products. Stem cell beauty technology and its related products have attracted extensive attention from beauty experts and industry insiders in the past two years. All stem cells, regardless of their origin (plant, animal or human) contain specific epigenetic factors as well as abundant nutrients that maintain the self-renewal capacity of stem cells. [0003] Plant stem cell technology is based on the wound recovery mechanism of plants. The plant is wounded to induce the formation of restored ti...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K8/98A61Q19/08A61Q19/00
Inventor 陈海佳王一飞葛啸虎马岩岩王小燕罗二梅舒辉萍
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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