Compound composition containing methylsulfonylconazole and triazole fungicide
A technology of mesycloconazole and compound composition, which is applied in the field of pesticides and can solve problems such as decline in disease prevention and control effects, unscientific drug use, and environmental pollution.
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Embodiment 1
[0219] The following is the combination of mesyconazole and difenoconazole, econazole, tebuconazole, hexaconazole, tetrafluconazole, diniconazole, carbenazole and flusilazole respectively against rice bacterial blight indoor toxicity test.
[0220] Bacterial solution preparation: pull out the cotton plug of the test tube near the alcohol lamp, pour 10ml of sterile water, and gently scrape the bacteria on the slope with a sterile inoculation needle to suspend the spores. Pour the spore suspension into a sterilized triangular flask with several glass balls in advance, shake it for 5 minutes, filter the bacterial solution with sterilized gauze, put it in another sterilized triangular flask, and then make the required bacterial solution.
[0221] Experimental method: melt the beef extract peptone agar medium, and when it is cooled to 45°C ~ 50°C, quickly pour it into a sterilized petri dish together with 0.2ml of bacterial suspension under aseptic conditions, 15ml of medium in eac...
Embodiment 2
[0250] The following is the indoor toxicity of mesyconazole combined with difenoconazole, econazole, myclobutanil, penconazole, triaconazole, bifendazole and cyproconazole respectively against citrus canker Determination test.
[0251] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press) and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006". In this test, the zone of inhibition method was used. On the aseptic operating table, pour the NA medium into the NA plate, and after drying, take 0.1ml of X. citri bacteria suspension and apply it evenly on the NA plate with a coating stick. After drying, punch a hole in the center of the plate with a hole puncher with a diameter of 7 mm, and then take 100 μl of the drug solution into the small hole. 5 replicates for each concentration. The treatment of adding sterile water to the small well was used as the blank control. After treatment, place them ...
Embodiment 3
[0276] The following is the indoor toxicity test of mesyconazole compounded with tebuconazole, hexaconazole, cloconazole and silfluazole to control cabbage soft rot.
[0277] Pull out the test tube cotton plug near the alcohol lamp, pour 10ml of sterile water, and gently scrape the bacteria on the slope with a sterile inoculation needle to suspend the spores, then pour the spore suspension Put it into a sterilized triangular flask with several glass balls in advance, shake it for 5 minutes, filter the bacterial solution with sterilized gauze, put it in another sterilized triangular flask, and then make the required bacterial solution.
[0278] Melt the beef extract peptone agar medium, and when it is cooled to 45°C-50°C, quickly pour it into a sterilized petri dish together with 0.2ml of bacterial suspension under aseptic conditions, 15ml of medium in each dish, shake well and cool down solidification. Use a sterilized hole puncher (aperture 4mm) to make a hole, drop 1ml of t...
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