Method for preparing and storing fruiting dunaliella algae paste

Abstract

The invention discloses a method for preparing and storing fruiting dunaliella algae paste, and belongs to the field of aquaculture. Preparation of fruiting dunaliella algae paste is mainly finished by algae fluid centrifugation of a centrifugal machine or algae cell precipitation of a single precipitant in the past, so that the preparation method is high in power consumption, high in cost, poor in sedimentation efficiency, long in sedimentation time and incomplete in precipitation. In order to overcome the defects in a traditional technology, the algae fluid is participated and concentrated by adopting the compound precipitant, so that the method is complete and fast in participation, low in expense, short in elapsed time, and simple and convenient to operate. A composite cryoprotectant is adopted, and a method for gradually cooling is adopted in the cryopreservation process of the algae paste, so that the method disclosed by the invention is simple in process, easy to grasp, small in harm to cells, and high in cell survival rate; the nutritional ingredients are not lost basically; and the storage time can be up to 5 years, and is much higher than 2-3 years of the traditional storage method.

Description

[0001] Technical field The present invention belongs to the field of aquaculture, and in particular relates to a method for making and preserving fruit-shaped Dunaliella algae paste. Background technique [0002] Dunaliella carpatica belongs to Chlorophyta, Chlorophyceae, Dunaliellaceae, Dunaliellaceae, Dunaliella genus, unicellular individual, pear-shaped, 8.8-12.5μm long, 6.5-11.3μm wide, with 1 at the front Equivalent to long flagella, with 1 nucleus in the cell, containing 1 pyrenoid cup-shaped chloroplast, with a red eye spot, no stretching vesicle. The cells often contain a large amount of β-carotene, causing the cells to be orange-red. Halophytic algae are found in many salt lakes, salt ponds and some high-salt water bodies. After domestication, they can grow and reproduce in ordinary sea water. Dunaliella fructiferum can reproduce rapidly in water bodies with suitable light, temperature and salinity, and accumulate a large amount of β-carotene. Under suitable condition...

AI Technical Summary

Problems solved by technology

The disadvantage is that the ultrafiltration membrane is easy to clog, it takes a lot of time to clean, and it cannot be mass-produced

This invention solves the problems of short storage period, easy pollution, troublesome operation, difficult control of storage conditions, easy failure, inconvenient transportation, and seasonal restrictions on supply of traditional liquid culture medium. , long storage time, no loss of nutrients, supply is not limited by seasons, low production cost, but the disadvantages are large investment in infrastructure, complicated process, high technical requirements for operators, and it is difficult for ordinary workers to master

[0005] In the past, the production of Dunaliella fruiting algae paste was mostly done by centrifuging the algae liquid or precipitating algal cells with a single precipitant, which either consumes a lot of electricity and costs a lot of money, or the precipitation effect is poor, the precipitation time is long and the precipitation is incomplete.

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