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Antrodia cinnamomea cultivation method

A cultivation method, the technology of Antrodia camphorata, which is applied in the field of edible fungus cultivation, can solve the problems of high preparation cost and miscellaneous bacteria contamination in the liquid fermentation method, and achieve the effects of simplifying the cultivation procedure, improving the quality and increasing the growth amount

Inactive Publication Date: 2015-04-01
ZHISHENG TIANJIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The liquid fermentation method in the prior art is to inoculate the mycelium of Antrodia Cinnamomea into a liquid culture solution. Although the liquid culture solution can increase the physiologically active substances contained in Antrodia Cinnamomea, it is necessary to add such as bagasse or whole grains during the cultivation process. The solid substrate serves as the support for the mycelium of Antrodia cinnamomea. Therefore, the liquid fermentation method must be cultivated in a large-volume reactor, and its preparation cost is high, and the liquid fermentation method is also prone to contamination by bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of Antrodia camphorata culture method, comprises the following steps:

[0016] 1) Bacteria bag production: Add water and enzymolysis to mulberry twigs, add compound enzyme preparation and mix well, ferment and enzymolysis at room temperature, add glucose, peptone, barley extract, yeast extract, curing agent in sequence after umelysis , the mixed solution is solidified into a column culture medium;

[0017] 2) Bacteria cultivation by inoculation: inoculate onto the column culture medium in step 1) under aseptic conditions, and then move into a constant temperature bacteria cultivation room for bacteria cultivation.

[0018] The parts by weight of each component are: 10 parts of glucose, 1 part of peptone, 10 parts of barley extract, 10 parts of yeast extract and 10 parts of curing agent.

[0019] The depth of the column medium in step 1) is 10 cm.

[0020] The inoculation site is 5 cm deep from the outer surface of the cylinder medium in step 1).

[0021] Bacte...

Embodiment 2

[0024] A kind of Antrodia camphorata culture method, comprises the following steps:

[0025] 1) Bacteria bag production: Add water and enzymolysis to mulberry twigs, add compound enzyme preparation and mix well, ferment and enzymolysis at room temperature, add glucose, peptone, barley extract, yeast extract, curing agent in sequence after umelysis , the mixed solution is solidified into a column culture medium;

[0026] 2) Bacteria cultivation by inoculation: inoculate onto the column culture medium in step 1) under aseptic conditions, and then move into a constant temperature bacteria cultivation room for bacteria cultivation.

[0027] The parts by weight of the components are: 25 parts of glucose, 3 parts of peptone, 25 parts of barley extract, 25 parts of yeast extract and 25 parts of curing agent.

[0028] The depth of the column culture medium in step 1) is 15 cm.

[0029] The inoculation site is 8 cm deep from the outer surface of the cylinder medium in step 1).

[00...

Embodiment 3

[0033] A kind of Antrodia camphorata culture method, comprises the following steps:

[0034] 1) Bacteria bag production: Add water and enzymolysis to mulberry twigs, add compound enzyme preparation and mix well, ferment and enzymolysis at room temperature, add glucose, peptone, barley extract, yeast extract, curing agent in sequence after umelysis , the mixed solution is solidified into a column culture medium;

[0035] 2) Bacteria cultivation by inoculation: inoculate onto the column culture medium in step 1) under aseptic conditions, and then move into a constant temperature bacteria cultivation room for bacteria cultivation.

[0036] The parts by weight of each component are: 50 parts of glucose, 5 parts of peptone, 50 parts of barley extract, 50 parts of yeast extract and 50 parts of curing agent.

[0037] The depth of the column culture medium in step 1) is 20 cm.

[0038] The inoculation site is 10 cm deep from the outer surface of the cylinder medium in step 1).

[0...

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Abstract

The invention provides an antrodia cinnamomea cultivation method. The antrodia cinnamomea cultivation method comprises the following steps: 1) bacterium bag manufacturing: adding water into mulberry branch powder, performing enzymolysis, adding a compound enzyme preparation into the mulberry branch powder, stirring uniformly, fermenting, performing the enzymolysis at room temperature, adding glucose, peptone, a barley extract, a yeast extract and a curing agent in sequence after the enzymolysis is performed, and solidifying a mixed solution to prepare a cylinder culture medium; 2) inoculating and culturing bacteria: inoculating an antrodia cinnamomea strain to the cylinder culture medium in the step 1) under an aseptic condition, and transferring the cylinder culture medium into a constant-temperature bacterium culture room for culturing the bacteria. The antrodia cinnamomea cultivation method has the beneficial effects that the growth amount of antrodia cinnamomea mycelia or fruiting bodies of a unit culture medium are improved; moreover, the situations of infectious microbe pollution can be reduced; the prior art is greatly improved.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a cultivation method of Antrodia camphorata. Background technique [0002] The liquid fermentation method in the prior art is to inoculate the mycelium of Antrodia Cinnamomea into a liquid culture solution. Although the liquid culture solution can increase the physiologically active substances contained in Antrodia Cinnamomea, it is necessary to add such as bagasse or whole grains during the cultivation process. The solid substrate serves as the support for the mycelia of Antrodia camphorata. Therefore, the liquid fermentation method must be cultivated in a large-volume reactor, and its preparation cost is high, and the liquid fermentation method is also prone to contamination by bacteria. Contents of the invention [0003] The problem to be solved by the present invention is to provide a method for cultivating Antrodia camphorata. [0004] For solving the ...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00
Inventor 季延滨李涛
Owner ZHISHENG TIANJIN BIOTECH
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